Abstract: Objective To analyze the advantages of spatial measurement of anatomical parameters in a 3D model in surgical planning for laparoscopic partial nephrectomy (LPN). Methods From February, 2016 to October, 2017, 37 patients diagnosed with T1 renal mass underwent LPN based on 3D reconstruction after enhanced CT scanning using the Uromedix-3D system (group A), and another 38 patients received LPN with conventional CT planning (group B). The anatomical parameters were measured in the reconstructed 3D model and the demographic data, surgical outcome and postoperative data were compared between the two groups. Results In group A, the average time for 3D model reconstruction was (29.3±9.7) min; the length, width and depth of the renal defect in 3D model were 3.2±1.1 cm, 2.6±0.9 cm and 1.7±0.7 cm, respectively; The distance of the tumor from the collecting system was 3.8±2.2 mm; The mean R.E.N.A.L score of the patients was 7±1.5, and 3 patients had accessory renal artery and 2 had early branching of the renal artery. LPNs were completed via the retroperitoneal approach in all the 75 patients without conversion to open or total nephrectomy. Group A and group B showed significant differences in warm ischemic time (26.7±6.4 vs 31.9±7.0 min), tumor-excision time (8.4±2.6 vs 10.4±2.8 min), renal defect suture time (18.3±3.9 vs 21.5±3.4 min), 24-h volume of retroperitoneal drainage (88.6±40.2 vs 134.3±58.3 mL) and 48-h volume of retroperitoneal drainage (127.9 ± 54.5 vs 198.1 ± 86.3 mL), but not in the demographic data, operation time, intraoperative blood loss or postoperative hospital stay. Conclusions 3D reconstruction of the renal masses can be completed efficiently and accurately using this system. Compared with conventional CT-based measurement, 3D spatial measurement of the anatomical structures helps to increase the precision in the performance of LPN and reduce the warm ischemia time.