南方医科大学学报

南方医科大学学报 ›› 2018, Vol. 38 ›› Issue (02): 168-.

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芍药苷对PM2.5诱导BEAS-2B细胞损害的保护作用

吴晓芳，王丽云，易建华，雷剑，奥宇宏，李建军，韩晶   

  • 出版日期:2018-02-20 发布日期:2018-02-20

Protective effect of paeoniflorin against PM2.5-induced damage in BEAS-2B cells

  • Online:2018-02-20 Published:2018-02-20

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摘要： ：目的探索芍药苷对PM2.5诱导人支气管上皮细胞（BEAS-2B细胞）损害的保护作用及其机制。方法采用析因设计，分别 利用MTT法，TBA法及化学荧光法，测定不同剂量芍药苷对PM2.5染毒所致BEAS-2B细胞生长抑制，细胞培养上清中丙二醛 （MDA）及细胞内活性氧（ROS）的含量的影响。结果随着PM2.5干预浓度水平的增高，细胞存活率显著性降低（P<0.05），并呈 剂量反应关系（r=-0.759，P<0.05），随着共处理芍药苷干预浓度的增高，PM2.5诱导BEAS-2B细胞存活率下降的幅度明显降低 （P<0.05），但未观察到剂量-反应关系（P>0.05）；同时发现PM2.5染毒可致细胞培养上清中MDA，细胞内ROS含量均显著增高 （P<0.05），芍药苷高浓度干预时，细胞培养上清中MDA，细胞内ROS含量较染毒对照组均显著性降低（P<0.05）。结论芍药苷 对PM2.5所致BEAS-2B细胞的生长抑制有明显的保护作用，其机制可能与芍药苷抗氧化作用有关。

Abstract: Objective To investigate the protective effects of paeoniflorin against PM2.5-induced damage in BEAS-2B cells and explore the possible mechanism. Methods With a factorial design, this study was performed to observe the protective effects of different doses of paeoniflorin against PM2.5-induced BEAS-2B cell growth inhibition and the effects of paeoniflorin on the contents of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) in the cell cultures. Results Exposure to increased PM2.5 concentrations caused significant decrease in the cell survival rate (P<0.05) with a clear dose-response relationship (r=-0.759, P<0.05). Treatment of the cells with paeoniflorin significantly attenuated PM2.5-induced inhibition of BEAS-2B cell survival (P<0.05), but the effect of paeoniflorin was not dose-dependent (P>0.05). PM2.5 exposure also significantly increased the contents of MDA and intracellular ROS (P<0.05), and paeoniflorin obviously antagonized these effects of PM2.5. Conclusion Paeoniflorin can protect BEAS-2B cells from PM2.5-induced growth inhibition, and the mechanism might be related to the anti-oxidant effects of paeoniflorin.