南方医科大学学报

南方医科大学学报 ›› 2017, Vol. 37 ›› Issue (07): 983-.

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FasL-844T/C基因多态性与退变腰椎间盘髓核FasL表达的关系

孙正明，彭毛加措，徐洪海，常彦海，吴学元，董向辉，凌鸣   

  • 出版日期:2017-07-20 发布日期:2017-07-20

Association of FasL-844T/C gene polymorphism with FasL expression in the nucleus pulposus of degenerative lumbar intervertebral discs

  • Online:2017-07-20 Published:2017-07-20

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摘要： 目的研究FasL-844T/C基因多态性、腰椎MRI和退变的腰椎间盘髓核组织中FasL表达之间的关系。方法采集105例腰 椎间盘突出症患者静脉血和髓核组织；利用基质辅助激光解吸附电离飞行时间质谱分析对FasL-844T/C基因多态性进行了检 测；采用S-P 法染色检测FasL在髓核组织中的表达。结果MRI研究发现，与CC基因型携带者相比，携带TT基因型的个体腰椎 间盘退变的积分显著增高（P=0.003）；与CC基因型携带者相比，TT基因型携带者髓核细胞表达FasL的积分有统计学差异（P= 0.048），但CT基因型携带者与CC基因型携带者之间髓核细胞表达FasL的积分无统计学差异（P=0.264）；腰椎间盘退变积分和 椎间盘髓核组织FasL表达无相关性。结论FasL-844T/C基因多态性与腰椎间盘突出症患者的椎间盘髓核组织FasL表达存在 相关性；腰椎间盘退变积分和椎间盘髓核组织FasL表达无相关性。

Abstract: Objective To investigate the association of FasL-844T/C gene polymorphism with the magnetic resonance imaging (MRI) findings and FasL expression in the nucleus pulposus of degenerative lumbar intervertebral discs. Methods Lumbar MRI data, venous blood and nucleus pulposus were collected from 105 patients with lumbar disc herniation. The genotypes of FasL-844T/C gene of the patients were determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Immunohistochemistry was used to detect the expression of FasL in the nucleus pulposus of the degenerative lumbar intervertebral discs. Results Compared with CC genotype, TT genotype of FasL-844T/C gene was associated with a significantly increased score of lumbar disc degeneration (P=0.003) as observed in MRI scan. FasL expression in the nucleus pulposus differed significantly between patients of FASL-844CC genotype and those of FASL-844TT genotype (P=0.048), but not between those of FASL-844CC and FASL-844CT genotypes (P=0.264). No significant association was found between MRI findings and FasL expression in the nucleus pulposus of the lumbar intervertebral discs. Conclusion FasL-844T/C gene polymorphism is correlated with the expression of FasL in the nucleus pulposus of the intervertebral disc in patients with lumbar disc herniation. MRI findings of the lumbar intervertebral discs do not correlate with the expression of FasL in the nucleus pulposus of the intervertebral discs.