南方医科大学学报

南方医科大学学报 ›› 2017, Vol. 37 ›› Issue (02): 199-.

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miRNA-140-5p在人骨髓间充质干细胞成脂分化中的表达及其靶基因的预测

汪涛，颜瑞巧，曹俊，曹玲玲，张铉浦，李兴暖，吴萍，周小鸥，吴建芳，许晓源   

  • 出版日期:2017-02-20 发布日期:2017-02-20

Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation

  • Online:2017-02-20 Published:2017-02-20

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摘要： 目的筛选出人骨髓间充质干细胞（hMSCs）成脂分化中具有潜在价值的miRNA及其靶基因，为人类成骨细胞与脂肪细胞 分化平衡调控机制的深入研究提供有力实验依据。方法收集hMSCs 成脂分化过程中的0、7、14、21 d 的细胞样品，应用 miRNA 芯片和转录组测序（mRNA-seq）技术，分析4个点细胞中miRNA和mRNA的表达水平，并将miRNA与mRNA的表达趋 势进行关联分析，聚焦具有研究价值的miRNA与mRNA相互调控关系，同时，采用TargetScan、PicTar和miRanda等数据库进行 靶基因的预测。结果发现miR-140-5p在成脂分化中表达量呈逐渐下降的趋势，而白血病抑制因子受体（LIFR）表达量呈逐渐 升高趋势，二者呈现负调控的对应关系。同时经3 个靶基因数据库预测，LIFR 为miR-140-5p 共有的靶基因。结论 miRNA-140-5p可能与人骨髓间充质干细胞的成脂分化密切相关，且通过调控其靶基因LIFR的表达而参与成脂分化。

Abstract: Objective To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation. Methods Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA. Results The expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p. Conclusion miRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.