南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (12): 1621-.

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雌激素通过调节AKT信号通路活性抑制肝癌细胞的侵袭和转移

田长印，张欣，赵文学，党双锁，靳耀锋，纪泛扑   

  • 出版日期:2016-12-20 发布日期:2016-12-20

Oestrogen inhibits invasion and metastasis of hepatocellular carcinoma MHCC97H cells by regulating the activity of AKT signaling pathway

  • Online:2016-12-20 Published:2016-12-20

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摘要： 目的探讨雌激素对MHCC97H肝癌细胞侵袭转移的作用及其相应机制。方法利用划痕试验检测雌激素对MHCC97H 肝癌细胞迁移能力的影响。Transwell小室法检测雌激素对MHCC97H肝癌细胞侵袭能力的影响。利用Western blotting法检 测雌激素对MHCC97H肝癌细胞中MMP-2和MMP-9 表达水平的影响。检测雌激素对MHCC97H肝癌细胞中AKT和p-AKT 蛋白表达水平的影响。结果雌激素能够有效抑制MHCC97H肝癌细胞的迁移和侵袭能力，随着浓度的升高抑制能力逐渐增 强，0.1 μmol/L和1 μmol/L浓度组MHCC97H肝癌细胞穿过基质胶到达小室底端的细胞数目分别为对照组的（68.99±15.74）% 和（34.28±8.17）%（P<0.05）。雌激素能够有效抑制MHCC97H肝癌细胞中MMP-2和MMP-9的表达，当浓度为1 μmol/L时，差 异具有统计学意义（P<0.05）。雌激素能够有效抑制MHCC97H肝癌细胞AKT的磷酸化水平，当浓度为1 μmol/L时，磷酸化水 平为对照组的（90±2）%（P<0.05）。结论雌激素能够有效抑制MHCC97H肝癌细胞的迁移和侵袭，这种作用可能部分与调节 AKT信号通路的活性进一步调节MMP-2和MMP-9的表达有关。然而，需要进一步的深入研究以证实这种作用关系。

Abstract: Objective To explore the inhibitory effect of estrogen against metastasis of human hepatocellular carcinoma MHCC97H cells and explore the molecular mechanism. Methods The inhibitory effect of estrogen on the migration and invasion of MHCC97H cells was evaluated with wound healing assay and Transwell assay. Western blotting was used for investigating the expression of MMP-2, MMP-9, AKT and p-AKT in the cells treated with estrogen. Results Estrogen treatment significantly inhibited the migration and invasion of MHCC97H cells in a dose-dependent manner. Estrogen significantly down-regulated the protein expressions of MMP-2 and MMP-9 and lowered the phosphorylation level of AKT. Conclusion The anti-metastatic effect of estrogen involves inhibition of MMP-2 and MMP-9 in MHCC97H cells probably by regulating AKT signal pathway.