南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (11): 1461-.

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过表达RIP140的肿瘤相关巨噬细胞抑制肝癌细胞的侵袭和增殖

易竹君，周赟，张文锋，陈浩，赵伟，刘作金，龚建平   

  • 出版日期:2016-11-20 发布日期:2016-11-20

Over-expression of receptor-interacting protein 140 in tumor-associated macrophages suppresses invasion and proliferation of hepatoma cells in vitro

  • Online:2016-11-20 Published:2016-11-20

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摘要： 目的探讨肿瘤相关巨噬细胞（TAMs）中RIP140 的表达对肝癌细胞侵袭、增殖的影响。方法慢病毒介导小鼠腹腔巨 噬细胞（PMs）RIP140 的过表达，Western blot 和Real-time PCR（qRT-PCR）分别检测PMs中RIP140 蛋白以及核酸表达水平， 流式细胞仪分析慢病毒转染率；Western blot、细胞免疫荧光和qRT-PCR 检测肝癌条件培养基（HCM）刺激PMs 后TAMs中 RIP140 的表达变化；HCM刺激PMs以及HCM刺激过表达RIP140 的PMs，qRT-PCR检测TAMs极化指标以及NF-κB和IL-6 的表达；Transwell 实验和细胞流式凋亡实验检测肝癌细胞的侵袭和凋亡；肝癌细胞和PMs以4∶1 比例注射于BALB/c裸鼠皮 下，建立裸鼠皮下肝癌模型，成瘤癌组织HE染色和免疫组化评定肝癌组织大体生长情况和肝癌细胞增殖能力。结果慢病 毒介导PMs RIP140 的过表达，病毒转染效率高，RIP140 过表达明显；HCM刺激PMs后，TAMs中RIP140 呈低表达；HCM诱 导TAMs呈M2 型极化，并且与肿瘤生长密切相关的NF-κB-IL-6 轴处于活化状态；TAMs可促进肝癌细胞侵袭和增殖，抑制 肝癌细胞凋亡。TAMs过表达RIP140 可抑制HCM介导的TAMs M2 型极化并抑制NF-κB/IL-6 通路的激活，减少IL-6 的释 放；除此之外，TAMs过表达RIP140 可抑制肝癌细胞的侵袭和增殖，促进肝癌细胞的凋亡。结论过表达RIP140 的TAMs可 抑制肝癌细胞的侵袭和增殖。其机制可能与TAMs过表达RIP140后抑制TAMs M2型极化有关。

Abstract: Objective To investigate the role of receptor-interacting protein 140 (RIP140) in tumor-associated macrophages (TAMs) in the invasion and proliferation of hepatoma cells in vitro. Methods Western blotting, qRT-PCR and flow cytometry were performed to examine the effects of lentivirus-mediated RIP140 over-expression in mouse peritoneal macrophages (PMs). Western blotting, qRT-PCR and immunofluorescence staining were used to detect the expression of RIP140 in TAMs following stimulation of the PMs with hepatocellular carcinoma conditioned medium (HCM) for 24 h. The polarization index and the expression of NF-κB and IL-6 were detected using qRT-PCR in TAMs in HCM-stimulated PMs with or without RIP140 over-expression. Transwell assay and flow cytometry were used to estimate the cell invasion and apoptosis. HE staining and immunohistochemical staining were used to analyze the effects of RIP140-over-expressing macrophages on the growth and tumor formation of H22 cells in BALB/c nude mice. Results The lentivirus vector efficiently mediated RIP140 over-expression in mouse PMs. HCM stimulation significantly inhibited RIP140 expression in the TAMs and promoted their M2-like polarization. Over-expression of RIP140 in PMs suppressed the invasion and induced apoptosis of HCC cells. RIP140 over-expression inhibited HCM-induced M2 polarization and the activation of NF-κB/IL-6 axis in the TAMs, and RIP140- overexpressing TAMs obviously suppressed the growth of H22 cell xenograft in nude mice. Conclusion Over-expression of RIP140 in TAMs suppresses the growth and proliferation of hepatoma cells possibly by inhibiting M2 polarization of the TAMs.