南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (11): 1456-.

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Pannexin1通道在顺铂诱导睾丸癌I-10细胞凋亡中的作用

巫剑峰，董淑英，武丹丹，陈银玲，童旭辉   

  • 出版日期:2016-11-20 发布日期:2016-11-20

Role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells

  • Online:2016-11-20 Published:2016-11-20

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摘要： 目的观察pannexin1通道在顺铂诱导睾丸癌I-10细胞凋亡中的作用及其机制。方法MTT法检测细胞存活率；Annexin V/PI双染法和Hoechst 33258染色法分别检测细胞早期和晚期凋亡；化学发光法检测细胞外ATP浓度；ELISA法检测细胞内IP3 含量。结果MTT法显示pannexin1通道抑制剂CBX与顺铂合用组的细胞存活率高于单用顺铂组（P<0.01）；Annexin V/PI双染 法显示CBX与顺铂合用组的细胞早期凋亡率低于单用顺铂组（P<0.001）；Hoechst 33258染色法显示CBX与顺铂合用组的细胞 晚期凋亡率低于单用顺铂组（P<0.01）；化学发光法表明CBX与顺铂合用组的细胞外ATP浓度低于单用顺铂组（P<0.05）；ELISA 法表明CBX与顺铂合用组的细胞内IP3浓度低于单用顺铂组（P<0.05）。结论Pannexin1通道参与顺铂诱导睾丸癌I-10细胞的 凋亡，其机制可能与介导ATP/IP3信号通路有关。

Abstract: Objective To investigate the role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells and the mechanisms. Methods MTT assay was used to assess the cytotoxicity of cisplatin (DDP) in I-10 cells. Annexin V/PI double staining and Hoechst 33258 fluorescence staining were employed to detect early- and late-stage apoptosis of the cells, respectively. Extracellular ATP level and intracellular IP3 level in the cells were detected using commercial detection kits. Results I-10 cells exposed to both CBX (a pannexin 1 channel inhibitor) and DDP showed a higher cell viability compared with the cells exposed to DDP alone (P<0.01). CBX significantly decreased cisplatin-induced early-stage apoptosis (P<0.001) and late-stage apoptosis (P<0.01), and cause obvious reductions in extracellular ATP and intracellular IP3 levels during cisplatin-induced apoptosis (P<0.05). Conclusion Pannexin 1 channels participate in cisplatin-induced apoptosis in I-10 cells possibly through the ATP/IP3 pathway.