南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (10): 1390-.

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ALDH1、CXCR4、E-cadherin在胃癌组织中的表达及与淋巴结转移的关系

赵艳，金鑫，李楠，李靖，钱军   

  • 出版日期:2016-10-20 发布日期:2016-10-20

Expression of ALDH1, CXCR4 and E-cadherin in castric carcinoma and their roles in lymphatic metastasis

  • Online:2016-10-20 Published:2016-10-20

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摘要： 目的探讨胃癌组织中ALDH1、CXCR4、E-cadherin 蛋白的表达及与淋巴结转移的关系。方法回顾性分析127例胃癌患 者临床病理资料及随访结果。免疫组化Elivision法检测胃癌标本及60例对照组织ALDH1、CXCR4、E-cadherin蛋白的表达情 况,进行统计分析，研究其对淋巴结转移的影响。结果ALDH1、CXCR4、E-cadherin 在胃癌组中的阳性率分别为57.5%（73/ 127）、63.8%（81/127）、36.2%（46/127），与对照组之间差异均有显著性（P<0.05）。随着患者临床分期越晚及淋巴结转移的出 现，ALDH1的阳性表达率升高（P<0.05）。CXCR4蛋白的表达与肿瘤的浸润深度、分化程度、临床分期和淋巴结转移相关（P< 0.05）。E-cadherin蛋白的表达与胃癌的浸润深度、分化程度及淋巴结转移密切相关（P<0.05）。3者在淋巴结转移阳性组表达率 均高于阴性组（P<0.05）；E-cadherin蛋白的表达与ALDH1、CXCR4蛋白的表达呈负相关关系，ALDH1与CXCR4蛋白的表达呈 正相关关系（P均<0.001）。生存分析表明，ALDH1、CXCR4阳性表达的患者生存率明显低于阴性者；E-cadherin蛋白表达水平 降低患者生存率明显下降（P<0.05）。多因素分析表明ALDH1、CXCR4和E-cadherin 的表达是影响胃癌患者预后的独立因素。 结论ALDH1，CXCR4表达的升高及E-cadherin表达的降低与胃癌的侵袭、转移和预后等因素相关，其联合表达与淋巴结转移 呈正相关，检测以上指标为预测胃癌的转移及预后评估提供依据。

Abstract: Objective To investigate the expression of ALDH1, CXCR4 and E-cadherin in gastric carcinoma and their roles in lymphatic metastasis. Methods Surgical specimens from 127 cases of gastric carcinoma were examined for expressions of ALDH1, CXCR4 and E-cadherin immuohistochemistry with 60 adjacent tissues as control. The associations of ALDH1, CXCR4 and E-cadherin with the clinicopathological pfeatures, 5-year survival rate and lymph node metastasis of the patients were analyzed. Results ALDH1, CXCR4 and E-cadherin were positive in 57.5% (73/127), 63.8% (81/127), and 36.2% (46/127) of the gastric carcinoma tissues, respectively, showing significant differences from the rates in the adjacent tissues (P<0.05). The expression of ALDH1 was significantly correlated with TNM stage and lymph node metastasis (P<0.05), CXCR4 was significantly correlated with the invasion depth, differentiation, TNM stage and lymph node metastasis of the tumor (P<0.05), and E-cadherin was significantly correlated with the invasion depth, differentiation and lymph node metastasis (P<0.05). The positivity rates of ALDH1, CXCR4 and E-cadherin were higher in cases with lymph node metastasis than in those without metastasis. E-cadherin expression was inversely correlated with ALDH1 and CXCR4 expression, and the latter two were positively correlated (P<0.001). Overexpressions of ALDH1 and CXCR4 and a decreased expression of E-cadherin were all related to a poor prognosis of the patients (P<0.05). The expressions ofALDH1, CXCR4 and E-cadherin were all independent prognostic factors of gastric carcinoma. Conclusion The expressions of ALDH1, CXCR4 and E-cadherin are associated with the invasion, metastasis and prognosis of gastric carcinoma, and their combined detection provides important evidence for predicting the progression and prognosis of gastric carcinoma.