南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (09): 1255-.

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丙泊酚下调H19抑制乳腺癌MDA-MB-231细胞的迁移和侵袭

白建杰，林春水，叶慧瑾，郭培培，汪威   

  • 出版日期:2016-09-20 发布日期:2016-09-20

Propofol suppresses migration and invasion of breast cancer MDA-MB-231 cells by down-regulating H19

  • Online:2016-09-20 Published:2016-09-20

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摘要： 目的观察丙泊酚对人乳腺癌MDA-MB-231细胞H19表达量的变化及其对迁移和侵袭能力的影响。方法体外培养人乳 腺癌MDA-MB-231 细胞，随机分为空白对照组（C组），DMSO组（S组），25 μmol/L 丙泊酚组（P1 组），50 μmol/L 丙泊酚组（P2 组），100 μmol/L丙泊酚组（P3组）。分别采用RT-PCR（TaqMan探针法）、划痕实验和Transwell小室法，检测丙泊酚对各组细胞 H19 表达量、细胞运动能力、细胞迁移和侵袭能力的影响。结果不同浓度的丙泊酚（25、50、100 μmol/L）处理24 h 后， MDA-MB-231 细胞的H19 表达量下调率分别为：17.83%、37.50%、63.67%（P<0.05）；细胞运动能力抑制率分别为：13.46%、 36.54%、46.17%（P<0.05）；细胞迁移抑制率分别为：27.93%、57.90%、76.51%（P<0.05）；细胞侵袭抑制率分别为：25.72%、 53.32%、81.43%（P<0.05）。结论丙泊酚可下调人乳腺癌MDA-MB-231细胞H19的表达，抑制其迁移和侵袭进程。

Abstract: Objective To explore the effect of propofol on H19 expression, migration and invasion of human breast cancer MDA-MB-231 cells in vitro. Methods MDA-MB-231 cells were randomly divided into 5 groups for treatment with basal medium, DMSO, or propofol at concentrations of 25, 50, and 100 μmol/L. H19 expression of the treated cells was assessed with RT-PCR, and the changes of cell motility, migration and invasion were evaluated with wound-healing assay and Transwell assays. Results Treatment of the cells with 25, 50, and 100 μmol/L propofol for 24 h down-regulated H19 by 17.83%, 37.50% and 63.67% (P<0.05), and suppressed cell motility by 13.46%, 36.54% and 46.17% (P<0.05), cell migration by 27.93%, 57.90% and 76.51% (P<0.05), and cell invasion by 25.72%, 53.32% and 81.43% (P<0.05), respectively. Conclusion Propofol-induced cell migration and invasion suppression are partially mediated by down-regulating H19 in MDA-MB-231 cells in vitro.