南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (04): 532-.

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Slug、ZEB1和KISS-1在胃腺癌中的表达及其临床意义

周蕾，胡永莲，武世伍，俞岚，承泽农，朱博   

  • 出版日期:2016-04-20 发布日期:2016-04-20

Expressions of Slug, ZEB1 and KISS-1 in gastric adenocarcinoma and their clinical significance

  • Online:2016-04-20 Published:2016-04-20

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摘要： 目的寻找能预测原发性胃腺癌（gastric adenocarcinoma，GAC）浸润、转移及预后的指标。方法采用免疫组织化学 ElivisionTM plus法检测261例GAC组织和80例正常胃黏膜组织中Slug、ZEB1和KISS-1蛋白的表达情况。结果在正常胃黏膜 组织中Slug、ZEB1和KISS-1蛋白的阳性表达率分别为2.5%，1.3%和87.5%；在GAC组织中阳性表达率分别为62.1%、29.1%和 45.2%，两组之间差异有统计学意义（P<0.05）。Slug在GAC组织中的阳性表达率与肿瘤组织的浸润深度、淋巴结转移及临床病 理分期均相关；ZEB1在GAC组织中的阳性表达率与分化程度、浸润深度、有无淋巴结转移及临床病理分期高低均相关；KISS-1 在GAC组织中的阳性表达率与浸润深度、淋巴结转移与否及临床病理分期均有关。Slug的表达和ZEB1的表达呈正相关关系； KISS-1与Slug和ZEB1呈负相关关系。Kaplan-Meier生存分析表明Slug和ZEB1蛋白阳性表达组患者总的生存时间明显低于 其阴性组患者；KISS-1 阳性表达组患者总的生存时间明显高于其阴性组患者。COX多因素模型分析显示，Slug、ZEB1 和 KISS-1蛋白的阳性表达以pTNM分期是影响GAC患者预后的独立因素。结论Slug、ZEB1和KISS-1的异常表达参与了GAC 的发生，并与GAC的淋巴结转移、pTNM分期及预后等均有关；Slug、ZEB1和KISS-1联合检测对GAC的进展及预后判断有重 要意义。

Abstract: Objective To identify potential markers for predicting invasion, metastasis, and prognosis of gastric adenocarcinoma (GAC). Methods The expressions of Slug, ZEB1 and KISS-1 were detected immunohistochemically in 261 GAC tissues and 80 normal gastric tissues. Results The positivity rates of Slug, ZEB1, and KISS-1 in gastric tissues were 2.5%, 1.3%, and 87.5%, respectively, significantly different from the rates of 62.1%, 28.4%, and 41.1% in GAC tissues (P<0.05). The expression level of Slug was significantly correlated with the depth of invasion, lymph node metastasis, and pTNM stages; the positivity rates of both ZEB1 and KISS-1 were significantly correlated with the tumor grade, depth of invasion, lymph node metastasis and pTNM stages. Slug expression was positively correlated with ZEB1 expression, and KISS-1 expression was inversely correlated with Slug and ZEB1 expressions. Kaplan-Meier analysis showed that the overall survival time of patients with positive expressions of Slug and ZEB1 was significantly shorter than that of the negative patients, and the survival time of patients positive for KISS-1 was significantly longer than the negative patients. COX multivariate analysis showed that positive Slug, ZEB1 and KISS-1 protein expressions and pTNM stages were independent prognostic factors of GAC (P<0.05). Conclusion The abnormal expressions of Slug, ZEB1 and KISS-1 may contribute to the tumorigenesis of GAC and are related with lymph node metastasis, pTNM stages, and prognosis of GAC. The combined detection of Slug, ZEB1, and KISS-1 expression has an important value in predicting the progression and prognosis of GAC.