南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (03): 297-.

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Expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma   

郝丽静，郑文娇，王淑芬，郑颖，何韶衡，张斌   

  • 出版日期:2016-03-20 发布日期:2016-03-20

LRG-1 在舌癌组织及舌癌细胞系Tca8113 中的表达及意义

  • Online:2016-03-20 Published:2016-03-20

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摘要： 目的探讨富亮氨酸α2糖蛋白1（LRG-1）在舌癌及舌癌细胞系Tca8113中的表达及其与舌癌临床病理参数的关系并分析
LRG-1的临床意义。方法采用S-P免疫组织化学方法检测我院40例浸润性舌癌患者的病灶及癌旁正常组织、20例舌部不典型
性增生组织、20例舌原位癌中LRG-1的表达，分析LRG-1表达与舌癌临床病理参数的相关性；流式细胞技术检测舌癌细胞系
（Tca8113）中LRG-1的表达；Western blotting法检测舌癌组织及细胞系Tca8113中LRG-1的表达；MTT法检测LRG-1对人血管
内皮细胞生长的影响；体外小管形成实验观察LRG-1对血管生成的影响。结果LRG-1阳性表达率在舌癌组织中（85%，34/40）
显著高于癌旁正常组织（10%，4/40），亦显著高于舌部不典型性增生组织（30%，6/20）及舌原位癌（50%，10/20），差异均有统计学
意义（P<0.05）。LRG-1在舌癌组织中的表达与患者年龄、性别等不相关，而与组织分化程度、临床分期及淋巴结转移有相关性
（P<0.05）。LRG-1能够促进血管内皮细胞的增殖和小管形成。结论LRG-1在舌癌组织及细胞系中表达异常，且能够促进血管
内皮细胞的生长和小管形成，提示其可能与肿瘤血管生成有关。

Abstract: Objective To investigate the expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma and
analyze the relationship between LRG-1 expression and the clinicopathological parameters. Methods LRG-1 expression was
detected in 40 tongue squamous cell carcinoma (TSCC) tissues and paired normal adjacent tissues, 20 atypical hyperplasia
tissues of the tongue, and 20 tissues of tongue cancer in situ using immunohistochemical method. The expression of LRG-1 in
Tca8113 cell line was detected using flow cytometry. The expression of LRG-1 was also detected in human TSCC tissues and
Tca8113 cells with Western blotting. The effect of LRG-1 on the proliferation of HUVECs was determined using MTT assay, and
its effect on angiogenesis was evaluated with Matrigel tube formation assays. Results Human TSCC tissues had a significantly
higher rate of positive expression for LRG-1 (85%, 34/40) than the adjacent tissues (10%, 4/40), invasive tongue cancer (30%, 6/
20), and tongue cancer in situ (50%, 10/20) (P<0.05). LRG-1 expression was correlated with the degree of tumor differentiation,
clinical stage and lymph node metastasis of the tumor (P<0.05) but not with the patients’ age or gender. In the in vitro
experiment, LRG-1 promoted HUVEC proliferation and angiogenesis. Conclusion Abnormal LRG-1 expression is present in
the human TSCC tissue and Tca8113 cells. LRG-1 can promote HUVEC proliferation and angiogenesis in vitro, suggesting its
possible role in promoting tumor angiogenesis.