南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (01): 94-.

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MT1-MMP在乳腺癌中的独特表达模式

姚广裕，陈路嘉，胡晓磊，李文姬，叶长生   

  • 出版日期:2016-01-20 发布日期:2016-01-20

Expression pattern of membrane type-1 matrix metalloproteinase in primary breast
carcinomas

  • Online:2016-01-20 Published:2016-01-20

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摘要： 目的探讨MT1-MMP在乳腺癌患者肿瘤组织中的表达特点。方法采用免疫组化、半定量RT-PCR和原位杂交方法分别
检测43例乳腺癌组织中MT1-MMP蛋白和mRNA的表达，研究MT1-MMP在乳腺癌组织中的表达特点。结果采用免疫组化
方法检测乳腺癌组织中MT1-MMP蛋白表达，发现阳性染色位于癌细胞胞膜；而采用原位杂交的方法检测到MT1-MMP mRNA
的阳性染色位于乳腺癌癌巢周围的间质细胞。采用半定量RT-PCR方法检测癌组织中MT1-MMP mRNA表达，所有标本均有
MT1-MMP mRNA的表达，其中MT1-MMP免疫组化阳性染色的乳腺癌组织中相对表达量高（0.759±0.0802）；在MT1-MMP免
疫组化阴性染色的乳腺癌中相对表达量低（0.547±0.0886），差异有统计学意义（P<0.01）。结论MT1-MMP 很可能是由乳腺癌
癌巢周围的间质细胞产生，激活后锚于癌细胞膜发挥作用，MT1-MMP在乳腺癌组织中的产生和作用有其独特的特点。

Abstract: Objective To examine the expression pattern of membrane type-1 matrix metalloproteinase (MT1-MMP) in breast
carcinomas. Methods Forty-three breast cancer tissues were collected and examined for MT1-MMP protein and mRNA
expressions using immunohistochemistry, semi-quantitative RT-PCR and in situ hybridization. Results Immunohistochemistry
of the breast cancer specimens showed MT1-MMP immunoreactivity on the cancer cell membrane. MT1-MMP mRNA was
located in the stromal cells surrounding the breast cancer nest as shown by in situ hybridization. MT1-MMP mRNA expression
was detected in all of the carcinomas, but its level was significantly lower in immunohistochemically negative specimens than
in positive ones (0.547 ± 0.0886 vs 0.759 ± 0.0802, P<0.01). Conclusion MT1-MMP is very likely produced by stromal cells
surrounding the breast cancer nest and anchored on the cell membrane after activation.