南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (11): 1649-.

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spvB/spvC基因对沙门菌毒力及宿主免疫功能的影响

刘晓艳，陈强，李红，朱春晖，吴春雪，王文杏，余晓君   

  • 出版日期:2015-11-20 发布日期:2015-11-20

Effect of spvB/spvC gene on salmonella virulence and the host immune function

  • Online:2015-11-20 Published:2015-11-20

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摘要： 目的研究spvB/spvC基因对沙门菌毒力及对宿主免疫系统的影响。方法野生型沙门菌（STM.211）、敲除spvB基因的沙门
菌（STM.211-ΔspvB）、敲除spvC 基因的沙门菌（STM.211-ΔspvC）、敲除spvB 及spvC 基因的沙门菌（STM.211-ΔspvB.spvC）和PBS
组分别经腹腔注射0.2 mL105 CFU的STM.211、STM.211-ΔspvB、STM.211-ΔspvC、STM.211-ΔspvB.spvC感染BALB/c小鼠，观察
小鼠精神状态、运动情况、腹泻、体质量、毛发改变等感染中毒症状，用ELISA法测定小鼠血清IL-10、IL-12、IFN-γ水平；并分离
小鼠肝、脾，观察靶器官大体观及显微镜下的病理改变。结果（1）STM.211、STM.211-ΔspvB和STM.211-ΔspvC组的中毒症状明
显较PBS 组严重，STM.211-ΔspvB.spvC 组与PBS 组间无明显差异；（2）STM.211 组、STM.211-ΔspvB 组、STM.211-ΔspvC 组的
IFN-γ和IL-12 分泌量均显著低于STM.211-ΔspvB.spvC 组（P<0.05），而IL-10 的分泌量明显高于STM.211-ΔspvB.spvC 组（P<
0.05）；STM.211组、STM.211-ΔspvB组、STM.211-ΔspvC组间差异无统计学意义（P>0.05）。结论（1）spvB/spvC基因缺失对沙门
菌毒力影响不明显，但spvB.spvC基因共同缺失，使沙门菌毒力减弱；（2）spvB/spvC基因相对抑制TH1细胞因子IFN-γ和IL-12的
分泌，促进TH2 细胞因子IL-10 的分泌，使免疫应答向TH2 方向偏移，从而有利于病原菌抵抗和逃避宿主的免疫防御，加重
感染结局。

Abstract: Objective To study the effect of spvB/spvC gene on Salmonella virulence and the Host immune. Methods STM.211,
STM.211-ΔspvB, STM.211-ΔspvC, STM.211-ΔspvB.spvC and PBS were infected with 0.2 mL 105 CFU corresponding strain
respectively by intraperitoneal. We observed the mental status, movement, diarrhea, weight, pelage changed hair of the
infected mouse. Then the level of IL-10, IL-12, IFN-γ were detected by ELISA. Finally, we observe the pathological changes of
liver and spleen with the general view and the microscope. Results Infection symptoms of STM.211, STM.211-ΔspvB and
STM.211-ΔspvC were significantly worse than PBS group, but there was no significant difference between STM.211-spvB.spvC
group and PBS group. The secretion of IFN-γ and IL-12 of STM.211, STM.211-ΔspvB, STM.211-ΔspvC group were significantly
lower than those in the STM.211-ΔspvB.spvC group (P<0.05), but IL-10 secretion was significantly higher than STM.211-ΔspvB.
spvC group (P<0.05). There were no statistical significance among the STM.211, STM.211-ΔSpvB, STM.211-ΔspvC groups (P>
0.05). Conclusion Salmonella virulence can be affected obviously by spvB combined with spvC gene, but not by spvB or spvC.
spvB/spvC gene can inhibit the TH1 cytokines (IFN-γ and IL-12) secretion but promote the TH2 cytokines (IL-10) expression,
leading immune response trend to TH2 shift. It shows that spvB/spvC gene can help the bacteria evade the host immune
defenses, leading to aggravation of infection.