南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (11): 1633-.

• • 上一篇    下一篇

肿瘤坏死因子-α对骨髓源性肥大细胞表达MMP-3、MMP-9、IL-17的影响

陈玉姣，欧阳晴晴，王然，毋静，赵进军，杨敏   

  • 出版日期:2015-11-20 发布日期:2015-11-20

Effects of tumor necrosis factor-α on release of MMP-3, MMP-9, and interleukin-17 in
mouse bone marrow-derived mast cells in vitro

  • Online:2015-11-20 Published:2015-11-20

HTML

PDF

1056

摘要： 目的肿瘤坏死因子-α（TNF-α）对骨髓源性肥大细胞（BMMCs）分泌MMP-3、MMP-9、IL-17的影响。方法原代培养小鼠
BMMCs，将培养8 周的BMMCs种植于12 孔板中。细胞分为4 个组：BMMCs+PBS组（对照组）、BMMCs+2 ng/mL TNF-α组
（2 ng/mL组）、BMMCs+10 ng/mL TNF-α组（10 ng/mL组）、BMMCs+50 ng/mL TNF-α组（50 ng/mL组），不同浓度TNF-α与BMMCs
作用后，在12、24 h收集细胞，用实时荧光定量逆转录聚合酶链式反应（real-time polymerase chain reaction, real-time PCR），在
mRNA水平检测MMP-3、MMP-9、IL-17 的表达。结果培养12 h 后，2 ng/mL 组、10 ng/mL 组、50 ng/mL 组MMP-3、MMP-9、
IL-17 mRNA的表达均高于对照组（P<0.05），并且随着TNF-α浓度的上升mRNA的表达显著升高（P<0.05）。24 h结果与12 h结
果一致，24 h 与12 h 之间比较，除50 ng/mL 组MMP-3 mRNA的表达无差异（P>0.05），其余各组之间MMP-3、MMP-9、IL-17
mRNA的表达显著升高（P<0.05）。结论TNF-α可以上调BMMCS表达MMP-3、MMP-9、IL-17，且具有浓度依赖性和时间依赖性。

Abstract: Objective To investigate the effect of tumor necrosis factor-α (TNF-α) on the release of matrix metalloproteinase-3
(MMP-3), MMP-9, and interleukin-17 (IL-17) in cultured mouse bone marrow-derived mast cells (BMMCs) in vitro. Methods
Primarily cultured mouse BMMCs at 8 weeks were exposed PBS (control) or TNF-α at the concentrations of 2, 10, or 50 ng/mL
for 12 or 24 h. Real-time PCR was performed to detect the mRNA expressions of MMP-3, MMP-9, and IL-17 in the exposed
cells. Results A 12-hour exposure of the BMMCs to TNF-α caused significantly increased expressions of MMP-3, MMP-9, and
IL-17 in a concentration-dependent manner (P<0.05). Prolonged exposures of the cells to 2 and 10 TNF-α for 24 h further
increased MMP-3, MMP-9, and IL-17 mRNA expressions, but exposure to 50 ng/mL TNF-α for 24 h increased only MMP-3 and
MMP-9 expressions but not IL-17 mRNA expression. Conclusions TNF-α treatment of primarily cultured BMMCs can
significantly increase the cellular expressions of MMP-3, MMP-9, and IL-17 mRNAin a time- and dose-dependent manner.