南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (11): 1624-.

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iR-100在上皮性卵巢癌顺铂耐药细胞中的表达及其与耐药的关系

郭鹏，彭冬先，熊翔鹏，张赛男   

  • 出版日期:2015-11-20 发布日期:2015-11-20

Expression of microRNA-100 and its correlation with drug resistance in human ovarian
cancer SKOV3/DDP cells

  • Online:2015-11-20 Published:2015-11-20

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摘要： 目的研究微小RNA-100（miR-100）在人上皮性卵巢癌顺铂耐药细胞中的表达及其与顺铂耐药的关系。方法脂质体
lipofectamine 2000介导成熟miR-100模拟物（mimics）及阴性对照片段（NC）、阻遏物（inhibitor）及阴性对照片段（inhibitor NC）
分别转染人卵巢癌顺铂耐药细胞株SKOV3/DDP。实验分为6 组：SKOV3 组、SKOV3/DDP 组、miR-100 mimics 组、NC组、
miR-100 inhibitor组及inhibitor NC组。实时荧光定量PCR及CCK8法分别检测各组细胞中miR-100的表达和顺铂半数抑制浓
度（IC50）。结果（1）SKOV3/DDP细胞的顺铂耐药指数（RI）为2.23；（2）miR-100在SKOV3/DDP细胞中低表达，与SKOV3相比
差异有统计学意义（P<0.001）；（3）转染miR-100 mimics 后，SKOV3/DDP细胞中miR-100 的表达水平与NC组相比上升38.29
倍，差异有统计学意义（P<0.01）；miR-100 mimics 组顺铂IC50与NC组相比明显降低，差异有统计学意义（P<0.001）；（4）转染
miR-100 inhibitor 后，SKOV3/DDP细胞中miR-100 的表达水平与inhibitor NC组相比下降97.7%（P<0.001）；miR-100 inhibitor
组顺铂IC50与inhibitor NC组相比明显升高（P<0.001）。结论miR-100在人卵巢癌顺铂耐药细胞中低表达，miR-100可增加卵
巢癌顺铂耐药细胞株对顺铂的敏感性，从而逆转其耐药。

Abstract: Objective To investigate the expression of microRNA-100(miR-100) and the relationship with cisplatin resistance in
human ovarian epithelial cancer SKOV3/DDP cells. Methods The SKOV3/DDP cells were transfected with the mimics or
inhibitor of miR-100 or negative control RNA (NC) or inhibitor negative control RNA (inhibitor NC) by lipofectamine 2000.
The experiment was divided into six groups: SKOV3 group, SKOV3/DDP group, miR-100 mimices group, NC group, miR-100
inhibitor group and inhibitor NC group. The expression of miR-100 and the cisplatin IC50 were measured by real-time PCR and
CCK8 assay respectively. Results（1）The cisplatin resistance index of SKOV3/DDP was 2.23;（2）The express level of miR-100
in SKOV3/DDP cells was significantly lower than that in SKOV3 cells (P<0.001);（3）After transfected with miR-100 mimics,
SKOV3/DDP cells showed that the level of miR-100 was 38.29 times higher than that in the NC group(P<0.01). The cisplatin IC50
of miR-100 mimices group was significantly lower than that in the NC group (P<0.001); (4) After transfected with miR-100
inhibitor, the level of miR-100 0f SKOV3/DDP was decreased by 97.7%. The cisplatin IC50 of miR-100 inhibitor group was
significantly increased as compared with that in the inhibitor NC group (P<0.001). Conclusion The expression of miR-100 is
downregulated in SKOV3/DDP cells. Overexpressing miR-100 may effectively increase the sensitivity to cisplatin of human
ovarian epithelial cancer SKOV3/DDP cells and may reverse cisplatin-resistance of EOC (epithelial ovarian cancer).