南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (10): 1390-.

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雷公藤甲素对佐剂关节炎肺功能降低大鼠Notch通路受体和配体基因表达的影响

万磊，刘健，黄传兵，谌曦，汪元，张皖东，刘磊，程园园，冯云霞   

  • 出版日期:2015-10-20 发布日期:2015-10-20

Effect of triptolide on expressions of Notch receptors and ligands in rats with adjuvantinduced
arthritis and reduced pulmonary function

  • Online:2015-10-20 Published:2015-10-20

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摘要： 目的观察雷公藤甲素（TPT）对佐剂关节炎（AA)大鼠Notch受体、配体表达的影响。方法将40只大鼠随机分为正常对照（NC）
组、模型（MC）组、甲氨喋呤（MTX）组、TPT组，每组10只，分别向除NC组外的其余动物右后足跖皮内注射弗氏完全佐剂0.1 mL
致炎，复制AA大鼠模型，致炎后第12填开始给药。NC组及MC组均给予生理盐水，其余组分别给予MTX、TPT。给药30 d后，检
测各组足趾肿胀度（E）、关节炎指数（AI）、肺功能、Notch受体/配体的变化。结果MC组大鼠E、AI、Notch3、Notch4、Delta1表达明
显升高；肺功能参数、Notch1、Jagged1、Jagged2表达降低（P<0.01）；与MC组比较，TPT组肺功能参数、Notch1、Jagged1、Jagged2的
表达升高，E、AI及Notch3、Notch4、Delta1表达降低，疗效优于对照组MTX（P<0.05，P<0.01）。结论TPT可能是通过上调Delta1、
Notch3、Notch4的表达、下调Jagged1、Jagged2、Notch1表达，降低炎症反应和免疫复合物沉积，改善AA关节和肺部症状。

Abstract: Objective To investigate the effects of triptolide on Notch receptor and ligand expressions in rats with
adjuvant-induced arthritis (AA). Methods Forty rats were randomly divided into normal control (NC) group, model (MC)
group, methotrexate group and triptolide groups. Rat models of AA were established by an intradermal injection of 0.1 mL
Freund’s complete adjuvant into the right paw. Twelve days after the injection, the rats were treated with corresponding drugs
for 30 days; the rats in NC group and MC group were given saline only. Paw edema volume (E), arthritis index (AI),
pulmonary function, histomorphologies, and Notch receptor/ ligand expression in the lung tissue were analyzed after the
treatments. Results Compared with the NC group, E, AI, Notch3, Notch4, and Delta1 expressions in the lung tissues
significantly increased while pulmonary function and pulmonary expressions of Notch1, Jagged1, and Jagged2 significantly
decreased the model rats (P<0.01). Compared with the MC group, triptolide-treated rats showed significantly improved
pulmonary functions, increased expressions of Notch1, Jagged1, and Jagged2 and decreased expressions of Notch3, Notch4,
and Delta1 in the lungs (P<0.05, P<0.01); the therapeutic effect of triptolide was better than that of methotrexate. Conclusion
Triptolide can reduce inflammatory reaction and immune complex deposition to improve joint and pulmonary symptoms in
rats with AA possibly by up-regulating the expressions of Notch3, Notch4, and Delta1 and down-regulating the expressions of
Jagged1, Jagged2, and Notch1.