南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (08): 1079-.

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鼠李糖乳杆菌抑制阪崎克罗诺杆菌致脑膜炎的体内外实验

钟亮，林如琴，龙北国，吴娴波，范宏英   

  • 出版日期:2015-08-20 发布日期:2015-08-20

Lactobacillus rhamnosus GG inhibits Cronobacter-induced meningitis in neonatal rats

  • Online:2015-08-20 Published:2015-08-20

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摘要： 目的考察鼠李糖乳杆菌（LGG）对阪崎克罗诺杆菌致脑膜炎的抑制作用。方法以Caco-2细胞为体外模型，采用黏附侵
袭实验研究阪崎克罗诺杆菌对Caco-2细胞的黏附侵袭作用及最佳感染时间，并用不同浓度的LGG与Caco-2细胞作用，确定其
抑制阪崎克罗诺杆菌的最佳浓度。进一步通过竞争、排斥和置换实验检测LGG对阪崎克罗诺杆菌黏附侵袭的抑制作用。通过
体内实验，研究LGG对乳鼠脑膜炎的作用效果。结果阪崎克罗诺杆菌对Caco-2细胞具有黏附侵袭性，最佳感染时间为3 h。
随着加入LGG 浓度的增加，阪崎克罗诺杆菌对Caco-2细胞的黏附和侵袭作用被显著抑制。LGG能通过竞争、排斥作用抑制阪
崎克罗诺杆菌对Caco-2细胞的黏附。LGG对阪崎克罗诺杆菌引起的脑膜炎具有预防和治疗作用，且预防效果更显著。结论通
过体内外实验，LGG可以抑制阪崎克罗诺杆菌进入肠屏障，从而减少其穿越血脑屏障的量，最终达到预防和治疗脑膜炎的效果。

Abstract: Objective To investigate the inhibitory effect of Lactobacillus rhamnosus GG ( LGG) against Cronobacter-induced
meningitis in neonatal rats. Methods The cell adhesion and invasion capacities of Cronobacter were assayed in Caco-2 cells,
and the optimal time length and concentration of the bacterium for infection were determined. The suppressive effects of LGG
on the adhesion and invasion of Cronobacter in caco-2 cells were tested by competitive and exclusion experiments, and its
inhibitory effect against Cronobacter-induced meningitis was evaluated in neonatal rats. Results Cronobacter showed aggressive
adhesion to caco-2 cells with an optimal infection time of 3 h. LGG produced a concentration-dependent inhibition of
Cronobacter adhesion and invasion by competing with and excluding the latter for cell adhesion. In neonatal rats, LGG showed
an obvious preventive effect and also a moderate therapeutic effect against Cronobacter-induced meningitis. Conclusion LGG
can inhibit Cronobacter entry across the intestinal barrier to achieve preventive and therapeutic effects against
Cronobacter-induced meningitis.