南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (07): 1073-.

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Anti-miR-145促进人气道平滑肌细胞增殖及骨桥蛋白合成

陈培芬，邱智辉，黄国华，张香梅，彭武建，曾辉，赖文岩   

  • 出版日期:2015-07-20 发布日期:2015-07-20

Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro

  • Online:2015-07-20 Published:2015-07-20

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摘要： 目的观察miR-145抑制剂（Anti-miR-145）对人支气管平滑肌细胞（HASMCs）的影响，探讨其在哮喘气道重塑中的作用。
方法分为对照组和实验组，实验组加入不同浓度的Anti-miR-145（10~100 nmol/L）。CCK-8法检测细胞增殖，流式细胞术检测
细胞凋亡，Western blotting 检测骨桥蛋白合成。结果与对照组比较，10 nmol/L和50 nmol/L Anti-miR-145显著促进HASMCs
增殖及骨桥蛋白合成（P<0.05或P<0.01），50 nmol/L Anti-miR-145 显著抑制HASMCs 凋亡（P<0.01）。结论Anti-miR-145通过
刺激HASMCs增殖和骨桥蛋白合成，抑制其凋亡，可能在哮喘气道重塑中发挥重要作用。

Abstract: Objective To investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and
osteopontin systhesis in vitro and explore the mechanisms. Methods HASMCs were treated with 10-100 nmol/L anti-miR-145,
and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes
in osteopontin synthesis after the treatment was quantified with Western blotting. Results Treatment with 10 and 50 nmol/L
anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L
anti-miR-145 obviously inhibited the cell apoptosis (P<0.01). Conclusion Anti-miR-145 promotes HASMC proliferation and
osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis
of airway remodeling.