南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (06): 816-.

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碱性亮氨酸拉链转录因子ATF样蛋白在狼疮小鼠肾脏组织中的表达及意义

彭宇声，黎 倩，王梦蕾，曹 灿，赖 宽，曾 抗   

  • 出版日期:2015-06-20 发布日期:2015-06-20

Expression of BATF, a member of the activator protein-1 family, in renal tissues of
MRL/lpr mice

  • Online:2015-06-20 Published:2015-06-20

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摘要： 目的探讨碱性亮氨酸拉链转录因子ATF样蛋白（basic leucine zipper transcription factor, ATF-like, BATF）在狼疮鼠肾脏
组织中的表达及临床意义。方法以24周龄雌性MRL/lpr小鼠作为狼疮肾炎模型组，同龄C57BL/6雌性小鼠作为正常对照组。
采用Western blot检测肾脏组织BATF的蛋白表达，采用RT-PCR检测肾脏组织中BATF、RORγt及IL-17 mRNA的表达水平，同
时检测小鼠尿蛋白、血清抗ds-DNA抗体以及肾脏组织病理的表现，并对BATFmRNA及RORγt、IL-17 mRNA相关性进行分
析。结果狼疮小鼠尿蛋白、血清抗ds-DNA抗体水平均高于正常小鼠（P<0.05）。狼疮小鼠肾脏组织病理主要表现为肾小球系
膜细胞增生，肾间质大量炎细胞浸润，而正常小鼠无明显异常。狼疮小鼠肾脏组织BATF mRNA和蛋白的表达量均较正常小鼠
升高（P<0.05）。狼疮小鼠肾脏组织RORγt、IL-17 mRNA表达量增多，且BATF mRNA的表达量与RORγt 、IL-17 mRNA表达量
呈正相关（r=0.945，0.876，P均<0.05）。结论在狼疮小鼠肾脏组织中BATF mRNA和蛋白的表达均高于对照组，BATF可能通过
上调Thl7免疫反应参与狼疮性肾炎的发病。

Abstract: Objective To investigate the expression of BATF, a member of the activator protein-1 family, in the renal tissues of
mice with lupus nephritis. Methods The renal tissues from 24-week-old female MRL/lpr mice and age- and sex-matched
C57BL/6 mice were examined for BATF protein expressions using Western blotting and for expressions of BATF, RORγt and
IL-17A mRNA using quantitative real-time PCR. The results of laboratory examinations were analyzed in relation with the
histopathology of the mice. Results The urinary protein and ds-DNA levels were significantly higher in MRL/lpr mice than in
the control mice (P<0.05). Compared to normal control mice, MRL/lpr mice showed obvious glomerular fibrosis and
inflammatory cell infiltrating with significantly increased BATF protein and mRNA expressions (P<0.05) and RORγt and IL-17
mRNA expressions in the renal tissues (P<0.05). In MRL/lpr mice, the expression of BATF mRNA was positively correlated
with the RORγt and IL-17A mRNA expressions in the renal tissues. Conclusion The renal expressions of BATF protein and
mRNA is increased in MRL/lpr mice. BATF may participate in the immunopathogenesis of lupus nephritis by enhancing Thl7
cell response.