南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (06): 801-.

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溶质转运体26A家族在HEK-293细胞中的表达和功能

范 超，傅鸣宇，肖中举，唐 杰   

  • 出版日期:2015-06-20 发布日期:2015-06-20

Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells

  • Online:2015-06-20 Published:2015-06-20

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摘要： 目的构建能在HEK-293细胞膜上表达的溶质转运体26A家族（SLC26A）的蛋白质粒，并对其生理功能进行检测。方法
构建多种人类SLC26A转运体－绿色荧光蛋白的融合质粒，并使用瞬时转染技术将其表达在人胚肾293细胞（HEK-293）中。用
全细胞膜片钳技术记录表达有SLC26A蛋白的HEK-293细胞的膜电容。结果每个SLC26A转运体均能在HEK-293细胞的细
胞膜上表达，并表现出和离子相互作用的生理功能。SLC26A转运体与离子结合的数目与其在细胞膜上的表达程度正相关并
具有一定的离子选择性。结论SLC26A转运体家族能够在HEK-293 细胞上表达并具有与转运离子结合的生理功能。在
HEK-293细胞系中表达SLC26A转运体家族的研究方法，为进一步研究此家族成员转运功能及其转运机制提供了可能。

Abstract: Objective To express solute carrier 26A proteins in HEK-293 cells and explore their functions. Methods
SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing
SLC26A proteins was measured by whole-cell patch recording. Results All the SLC26A transporters were expressed on the
membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which
represented their binding capability with anions. Conclusion The SLC26A transporters expressed on HEK cells show similar
functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A
transporters.