南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (05): 619-.

• •    下一篇

灵芝酸A对人骨肉瘤细胞增殖、凋亡和迁移的影响

邵建立，李志忠，焦根龙，孙国栋，周志刚   

  • 出版日期:2015-05-20 发布日期:2015-05-20

Ganoderic acid A suppresses proliferation and invasion and
induces apoptosis in human osteosarcoma cells

  • Online:2015-05-20 Published:2015-05-20

HTML

PDF

1843

摘要： 目的探讨灵芝酸A对骨肉瘤细胞增殖、凋亡和迁移的影响。方法体外培养人骨肉瘤HOS和mg-63细胞与0.1、0.25 和
0.5 mmol/L的灵芝酸A孵育，采用CCK8检测HOS和MG-63细胞的增殖，流式细胞仪检测细胞凋亡，Transwell检测细胞的迁移
和侵袭。Western blot 检测细胞中p-STAT3、STAT3、p-p38、p38和NF-ΚB1蛋白的表达变化。结果灵芝酸A能够显著抑制人类
骨肉瘤HOS和MG-63细胞的增殖，促进细胞凋亡以及抑制细胞迁移，并且这种效应存在剂量依赖性。与0.5 mmol/L 灵芝酸A
孵育能够显著降低HOS和MG-63细胞中STAT3的磷酸化水平，增加p38磷酸化水平，同时增加NF-κB1的表达水平。结论灵
芝酸A能够杀死人类骨肉瘤HOS和MG-63细胞，可以作为抗骨肉瘤的药物进行开发。

Abstract: Objective To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma
cells in vitro. Methods MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell
proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The
expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting. Results GA-A effectively inhibited the
proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis
in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of
Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the
phosphorylation level of p38 and NF-κB1 expression in both cells. Conclusion GA-A can induce proliferation inhibition,
apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.