南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (03): 338-.

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miR-223在肾透明细胞癌中的表达与功能

牛少曦，马鑫，张瑜，巩会杰，高宇，李新涛，沈东来，王雷，姚远新，张旭   

  • 出版日期:2015-03-20 发布日期:2015-03-20

Expression of miR-223 in clear cell renal cell carcinoma and its significance

  • Online:2015-03-20 Published:2015-03-20

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摘要： 目的探讨miR-223在肾透明细胞癌中的表达与功能。方法用real-time RT-PCR法检测miR-223在肾透明细胞癌组织和
瘤旁组织中的表达以及细胞系中miR-223的表达量。用transwell迁移实验和划痕实验测转染miR-223模拟物的786-O细胞的
迁徙能力，MTS法测其增殖情况，流失细胞仪分析其细胞周期。并用SPSS统计软件分析其临床数据与miR-223 表达量的关
系。结果miR-223在肾透明细胞癌组织和细胞系中的表达量高于瘤旁组织和正常肾小管上皮细胞（P=0.0003），其miR-223的
表达量与肿瘤的大小有关，肿瘤直径大于4 cm的肿瘤miR-223 表达量升高（P=0，0028）。转染miR-223 模拟物的786-O 细胞
transwell细胞迁移数比对照组多（P<0.0001），划痕实验显示实验组愈合能力比对照组快，增殖实验表明实验组增殖速度快于对
照组（P=0.006），细胞周期显示实验组细胞G1期减少，S期细胞增加，提示处于增殖的细胞数多（P<0.05）。结论miR-223可能
起着促进肾透明细胞癌发生发展的作用，可能是抗肾透明细胞癌治疗的新的抗癌靶标。

Abstract: Objective To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical
implications. Methods Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc,
pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay
were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was
used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry.
Results Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was
associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<
0.0001) and growth (P=0.006) and induced G1 cell cycle arrest. Conclusion miR-223 promotes renal cancer cell migration and
proliferation and may serve as a potential therapeutic target for ccRcc.