南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (02): 284-.

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瞬时感受器电位通道1在臭氧致小鼠肺组织炎症中的表达

扶招弟，周丽芬，黄建荣，郭淑仪，章洁纯，方永标，刘筱蔼，陈庆梓，李建华   

  • 出版日期:2015-02-20 发布日期:2015-02-20

Expression of transient receptor potential canonical 1 in ozone-induced inflammatory
lung tissues in mice

  • Online:2015-02-20 Published:2015-02-20

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摘要： 目的探讨经典瞬时感受器电位通道1（TRPC1）在臭氧暴露下小鼠肺组织中的表达情况。方法采用臭氧暴露建立小鼠肺
组织炎症模型，通过对小鼠行为学观察、支气管肺泡灌洗液的细胞分类计数和肺组织病理形态分析证实模型建立成功后，
RT-PCR法检测肺组织TRPC1 mRNA的表达，Western blot法和免疫组织化学染色法分别观察TRPC1蛋白在肺组织的表达和
定位情况。结果RT-PCR和Western blot 结果显示，与对照组相比，臭氧组小鼠肺组织TRPC1 mRNA及其蛋白表达上调（P<
0.05）；免疫组织化学染色法进一步证实，臭氧组小鼠肺组织TRPC1蛋白在肺泡上皮细胞、支气管上皮细胞、浸润的炎症细胞的
表达均较对照组明显增强（P<0.05）；相关性分析显示，小鼠肺组织中TRPC1 mRNA和蛋白的表达均与支气管肺泡灌洗液中白
细胞总数、巨噬细胞、中性粒细胞、淋巴细胞数呈显著性正相关（P<0.01）。结论TRPC1可能与小鼠肺组织炎症发病机制有关。

Abstract: Objective To detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of
ozone-induced lung inflammation and explore its role in lung inflammation. Methods In a mouse model of lung inflammation
established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern
blotting and immunohistochemistry. Results Compared to the control mice, the mice exposed to ozone showed significantly
increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry
showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells
in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with
the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed
mice (P<0.01). Conclusion TRPC1 may play a role in ozone-induced lung inflammation in mice.