南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (02): 239-.

• • 上一篇    下一篇

Src 激酶抑制剂PP2对星形胶质细胞缺氧/复氧损伤的保护作用

谷昱琛，童旭辉，于丽，焦浩，余彬彬，董淑英   

  • 出版日期:2015-02-20 发布日期:2015-02-20

Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro

  • Online:2015-02-20 Published:2015-02-20

HTML

PDF

1569

摘要： 目的探讨Src激酶抑制剂PP2对大鼠星形胶质细胞缺氧/复氧损伤的保护作用。方法实验分为正常对照组，缺氧/复氧组
（H/R；缺氧8 h/复氧24 h），PP2 +缺氧/复氧组（PP2+H/R；缺氧前给予10 μmol/L PP2作用24 h）。MTT法检测各组星形胶质细胞
存活率，流式细胞术测定各组星形胶质细胞凋亡率，Western blotting法检测各组星形胶质细胞中Src激酶、Bax及Bcl-2的蛋白
表达。结果MTT检测结果显示H/R 损伤后细胞存活率降低，而在使用PP2预处理后细胞存活率增加（P<0.01）；流式细胞术实
验结果显示H/R 损伤后细胞凋亡增加，而在使用PP2预处理后细胞凋亡显著减少（P<0.01）；Western blotting法检测结果显示H/
R 损伤后Src激酶表达增加，用PP2预处理后Src激酶表达降低。H/R 损伤后凋亡相关蛋白Bax/Bcl-2的比值增加，在用PP2预
处理后Bax/Bcl-2的比值显著降低（P<0.01）。结论PP2可对星形胶质细胞H/R损伤起保护作用，其机制可能与抑制Src激酶的
蛋白表达和激活抗凋亡机制有关。

Abstract: Objective To investigate the effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation (H/R) injury in rat astrocytes
in vitro. Methods In vitro cultured rat astrocytes were exposed to hypoxia for 8 h followed by reoxygenation for 24 h with or
without pretreatment with PP2 (10 μmol/L) for 24 h before H/R injury. MTT assay and flow cytometry were used to detect the
viability and apoptosis of the exposed astrocytes, respectively, and the protein expressions of Src, Bax, and Bcl-2 in the cells
were determined using Western blotting. Results PP2 pretreatment significantly increased the viability and decreased the
apoptosis rate of rat astrocytes exposed to H/R injury (P<0.01). Western blotting showed that H/R injury caused increased
expression of Src kinase, which was lowered by PP2 pretreatment. The ratio of Bax/bcl-2 in the astrocytes increased after H/R
injury, and was significantly decreased by PP2 pretreatment (P<0.01). Conclusion PP2 protects rat astrocytes from H/R injury
possibly by inhibiting the expression of Src kinase and activating the anti-apoptotic mechanisms in the cells.