南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (01): 72-.

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四甲氧基二苯乙烯抑制多潜能干细胞C3H10T1/2的脂肪分化

范翠芳，朱安娜，黄婷婷，李露，王素青   

  • 出版日期:2015-01-20 发布日期:2015-01-20

Tetramethoxystilbene, a selective CYP1B1 inhibitor, suppresses adipogenesis of C3H10T1/
2 pluripotent stem cells

  • Online:2015-01-20 Published:2015-01-20

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摘要： 目的探讨不同浓度CYP1B1抑制剂TMS（2,3’,4,5’,-四甲氧基二苯乙烯）对于C3H10T1/2多潜能干细胞脂肪分化及相关
基因表达的影响。方法体外培养C3H10T1/2细胞株至完全融合后，接触抑制2 d，用激素刺激混合物（IDM）（10 μg/ml胰岛素，
2 μmol/L地塞米松和0.5 mmol/L 3-异丁基1-甲基黄磦呤）诱导分化，同时加入不同浓度TMS（0，1.0，2.0、4.0 μg/ml）。观察各组
细胞分化程度，脂肪分化关键转录核因子——过氧化物酶体增殖物活化受体（PPARγ）及其下游靶基因CD36、脂肪酸结合蛋白4
（FABP4）的的表达状况。结果油红和TG含量测定结果显示，CYP1B1选择性抑制剂TMS可剂量依赖地抑制IDM诱导的多潜
能干细胞C3H10T1/2向脂肪细胞分化；这一作用源于TMS抑制转录核因子PPARγ的转录和蛋白表达以及下游靶基因CD36与
FABP4表达。结论TMS抑制由IDM诱导的多潜能干细胞C3H10T1/2向脂肪细胞分化。

Abstract: Objective To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic
differentiation of C3H10T1/2 multi-potent mesenchymal cells. Methods In vitro cultured C3H10T1/2 cells at full confluence
were induced by adipogenic agents (10 μg/ml insulin, 2 μmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine)
and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0 μg/ml. Oil Red-O staining was used to observe
the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes
cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western
blotting. Results Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2
cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear
transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4.
Conclusion TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ.