南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (01): 149-.

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重复性低氧预处理对大鼠肾缺血再灌注所致肝脏损伤的影响

鄢娜，冯泽国，颜光涛，岳剑虹，赵艳军，耿娜   

  • 出版日期:2015-01-20 发布日期:2015-01-20

Effect of repeated hypoxic preconditioning on renal ischemia-reperfusion-induced
hepatic dysfunction in rats

  • Online:2015-01-20 Published:2015-01-20

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摘要： 目的探究重复性低氧预处理（RHP）对大鼠肾脏缺血再灌注损伤（IRI）所致的肝脏损伤的影响并初步探讨其机制。方法
120只雄性SD大鼠随机分为4组（n=30）：低氧预处理手术组（RHP组），低氧预处理假手术组（RHPS组），常压手术组（IRI组）及
常压假手术组（S组）。低压氧舱预处理大鼠5 d，建立肾IRI模型，RHP组及IRI组统一切除右肾、夹闭左肾肾门45 min后放开建
立肾缺血再灌注模型，而RHPS组及S组仅切除右肾，不进行左肾缺血再灌注处理。于再灌注后2、8、24 h检测血清谷丙转氨酶
（ALT）、IL-17A、TNF-α浓度，酶标仪检测肝脏匀浆超氧化物歧化酶（SOD）及一氧化氮（NO）的含量，Western blot 检测测肝脏
p-PI3K、p-AKT水平，病理组织形态学检查观察肝脏结构变化。结果与IRI组相比，RHP组大鼠再灌注后2、8、24 h病理组织形
态学检查显示损伤减轻，血清ALT浓度降低，TNF-α水平于再灌注后24 h降低（P<0.05）肝组织NO含量升高（P<0.05），SOD含
量于再灌注后8 h升高（P<0.05）；与S组相比，IRI组及RHP组血清IL-17A浓度均显著升高（P<0.05），但两组间差异无统计学意
义（P>0.05）；RHP组P-PI3K及P-AKT表达均高于IRI组（P<0.05），且差异于再灌注后8 h尤为显著（P<0.05）。结论RHP对大
鼠肾脏IRI所致的肝脏损伤具有保护作用，但并非通过抑制IL-17A来实现。

Abstract: Objective To explore the effect of repeated hypoxic preconditioning (RHP) on renal ischemia-reperfusion-induced
hepatic dysfunction in rats and the underlying mechanism. Methods A total of 120 normal SD rats were randomly divided into
4 groups (n=40), namely RHP surgical group, RHP sham-operated (RHPS) group, nonhypoxic surgical group (IRI group), and
nonhypoxic sham-operated group (S group). The rats in the hypoxic groups were exposed to hypoxia in a hypoxic chamber for
5 days prior to establishment of renal ischemia-reperfusion model by resection of the right kidney and clamping the left renal
hilum. Serum alanine aminotransferase (ALT), IL-17A, TNF-α, liver superoxide dismutase (SOD) and nitric oxide (NO) were
detected at 2, 8 and 24h after reperfusion, and Western blotting was used to determine the expression of p-PI3K and p-AKT;
HE staining was used to observe the structural changes in the liver. Results Compared with IRI group, RHP group showed
significantly milder hepatic damage, lower ALT levels and higher NO levels at 2, 8, and 24 after reperfusion (P<0.05); TNF-α
levels were lowered at 24 h (P<0.05) and SOD increased at 8 h after the reperfusion (P<0.05). Compared with S group, IRI
group and RHP group showed significantly higher IL-17A levels (P<0.05) but without significant difference between the latter
two groups (P>0.05). The expressions of p-PI3K and P-Akt in RHP group were significantly higher than those in IRI group (P<
0.05), especially at 8 h after reperfusion (P<0.05). Conclusion Repeated hypoxic preconditioning can attenuate hepatic injury
induced by renal ischemia-reperfusion injury in rats.