Abstract: Objective To investigate the property of genipin-crosslinked silk fibroin(SF)/chitosan(CS) microspheres for slow
releasing of bovine serum albumin (BSA). Methods BSA-loaded genipin-crosslinked SF/CS microspheres were prepared by
emulsion cross-linking technique. The micropheres were observed for surface morphology and size distribution under
scanning electron microscope (SEM), and X-ray diffractometry (XRD) and fourier transform infrared spectroscopy (FTIR) were
used to analyze their structural characteristics. BCA method was used for determining the drug entrapment, loading rate and
cumulative drug release in 21 days. Results The microspheres were spherical and showed a smooth surface with an average
diameter of 7.84±0.97 μm. The drug entrapment efficiency of the microspheres was (50.16±4.32)% with a drug loading ratio of
(1.25 ± 0.11)% and a cumulative release of the total drug of (75.2 ± 2.53)% in 21 days. Conclusion Genipin-crosslinked SF/CS
microspheres have a high drug entrapment efficiency and possess good capacity of sustained drug release.