南方医科大学学报

南方医科大学学报 ›› 2024, Vol. 44 ›› Issue (4): 787-794.doi: 10.12122/j.issn.1673-4254.2024.04.22

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芡实壳提取物的抗氧化活性及对口腔溃疡模型大鼠的治疗作用

王 琼，许凤清，邓梦云，任梦婷，王桐生，吴德玲   

  1. 安徽中医药大学药学院，中西医结合学院，安徽 合肥 230012；中药研究与开发安徽省重点实验室，中药饮片制造新技术安徽省重点实验室，安徽 合肥 230012
  • 发布日期:2024-04-29

Antioxidant activity of Euryale ferox seed shell extract and its therapeutic effects on oral ulcer in rats

WANG Qiong, XU Fengqing, DENG Mengyun, REN Mengting, WANG Tongsheng, WU Deling   

  1. School of Pharmacy, School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230012, China; Anhui Provincial Key Laboratory of Chinese Medicine Research & Development, Anhui Provincial Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces, Hefei 230012, China
  • Published:2024-04-29

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摘要： 目的 研究芡实壳对口腔溃疡模型大鼠的防治作用及其潜在作用机制。方法 分别以福林酚法、硝酸铝比色法测定芡实壳萃取物中多酚、黄酮含量；以DPPH·、ABTS+·、·OH和·O2-4种自由基清除实验评价体外抗氧化活性；冰醋酸灼伤法建立口腔溃疡大鼠模型。采用酶联免疫法检测血清中氧化因子水平；HE染色观察溃疡黏膜组织病理变化；Western blot方法检测溃疡黏膜组织中Keap1、Nrf2、Nes-Nrf2和HO-1蛋白表达水平。结果 乙酸乙酯萃取物中多酚、黄酮含量为306.74±1.04 mg/g、23.43±0.61 mg/g，其清除DPPH·、ABTS+·自由基的IC50分别是3.42±0.97 µg/mL、3.32 ± 0.90 µg/mL（P<0.01）；乙酸乙酯组能明显改善模型动物口腔黏膜损伤，提高血清中CAT水平、降低MDA水平（P<0.01），显著增加血清中NEs-Nrf2（P<0.01）以及HO-1（P<0.05）蛋白表达量、降低Keap1（P<0.01）蛋白表达量显著。结论 芡实壳提取物对口腔溃疡有一定治疗作用，其作用机制可能是通过激活Keap1/Nrf2/HO-1信号通路。

关键词: 芡实壳；抗氧化活性；口腔溃疡；Nrf2；HO-1；Keap1

Abstract: Objective To investigate the therapeutic effect of Euryale ferox seed shell extract on oral ulcer in rats and its underlying mechanism. Methods The contents of polyphenols and flavonoids in Euryale ferox seed shells were determined by Folin-phenol assay and aluminum nitrate colorimetry, respectively. DPPH·, ABTS+·, ·OH and ·O2- scavenging experiments were performed to evaluate the antioxidant activities of Euryale ferox seed shell extract in vitro. In a rat model of oral ulcer induced by burning with glacial acetic acid, the therapeutic effect of Euryale ferox seed shell extract was assessed by detecting changes in serum levels of oxidative factors by enzyme-linked immunosorbent assay (ELISA) and observing pathological changes of the ulcerous mucosa using HE staining; the therapeutic mechanism of the extract was explored by detecting the expression levels of Keap1, Nrf2, Nes-Nrf2 and HO-1 proteins in ulcerous mucosa using Western blotting. Results The ethyl acetate extract of Euryale ferox seed shells contained 306.74±1.04 mg/g polyphenols and 23.43±0.61 mg/g flavonoids and had IC50 values for scavenging DPPH · and ABTS + · free radicals of 3.42 ± 0.97 µg/mL and 3.32 ± 0.90 µg/mL, respectively. In the rat models, the ethyl acetate extract significantly ameliorated oral mucosal ulcer, increased serum CAT level, and decreased serum MDA level. The protein expression levels of Nes-Nrf2 and HO-1 were increased and Keap1 protein expression was lowered significantly in the ulcerous mucosa of the rats after treatment with the extract (P<0.05 or 0.01). Conclusion The therapeutic effect of Euryale ferox seed shell extract on oral ulcers in rats is mediated probably by activation of the Keap1/Nrf2/HO-1 signaling pathway.

Key words: Euryale ferox seed shell extract; antioxidant activity; oral ulcer; Nrf2; HO-1; Keap1