南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (08): 1207-1212.doi: 10.12122/j.issn.1673-4254.2020.08.20

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不同结扎材料对慢性压迫性损伤模型胶质细胞活化的影响

王 琛，陈 鹏，林东升，陈 弋，吴智兵，林兴栋   

  • 出版日期:2020-08-20 发布日期:2020-08-20
  • 基金资助:

Effects of different materials for partial sciatic nerve ligation on glial cell activation in rat models of chronic constriction injury

  

  • Online:2020-08-20 Published:2020-08-20

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摘要： 目的 探究不同结扎材料对慢性压迫性损伤（CCI）模型行为学表现、脊髓胶质活化及相关炎症因子表达的影响。方法 44只SD大鼠随机分为对照组（n=15）、丝线CCI组（n=15）和铬线CCI组（n=14），术后3、7、11、15 d检测大鼠损伤侧机械缩足反射阈值（MWT）及热缩足反射阈值（TWL）。并采用特殊染色、Western blot法及RT-PCR法检测大鼠损伤部位病理改变、胶质细胞及炎性细胞因子的表达。结果 术后3~15 d，丝线组与铬线组的MWT和TWL水平较对照组显著降低（P<0.05），术后3 d铬线组的TWL较丝线组出现显著降低（P<0.05）。坐骨神经染色结果显示丝线组与铬线组均出现了神经干损伤及脱髓鞘改变。Western blot法及RT-PCR法检测结果提示丝线组与铬线组大鼠小胶质细胞标记物（CD11b）及胶质纤维状酸性蛋白（GFAP），以及IL-1β 及TNF-α mRNA表达水平接近，并显著高于对照组（P<0.05），而铬线组IL-6 mRNA表达高于丝线组（P<0.05）。结论 丝线和铬线制备的CCI模型均产生了胶质细胞激活作用，其中铬线组的炎症反应更加强烈。

Abstract: Objective To compare the effects of different materials for partial sciatic nerve ligation on glial cell activation in the spinal cord in a rat model of chronic constriction injury (CCI). Methods SD rats were randomly divided into the sham group (n=15), silk suture CCI group (n=15) and chromic catgut CCI group (n=14). The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of the rats were detected at 3, 7, 11 and 15 days after the operation. The changes in the sciatic nerve, the activation of spinal cord glial cells and the expression of inflammatory factors were observed using Western blotting and RT-PCR. Results At 3 to 15 days after the surgery, MWT and TWL of the rats were significantly lower in silk suture group and chromic catgut group than in the control group (P<0.05), and was significantly lower in chromic catgut group than in the silk suture group (P<0.05) at 3 days after the surgery. The results of sciatic nerve myelin staining showed that the sciatic nerve was damaged and demyelinated in both the ligation groups. The expressions of CD11b, GFAP, IL-1β and TNF-α in the two ligation groups were similar and all significantly higher than those in the control group (P<0.05). IL-6 mRNA level was significantly higher in chromic catgut group than in the silk suture group (P<0.05). Conclusion The CCI models established by partial sciatic nerve ligation with silk suture and chromic catgut all show glial activation, and the inflammatory response is stronger in chromic catgut group.