南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (07): 936-941.doi: 10.12122/j.issn.1673-4254.2020.07.04

• • 上一篇    下一篇

子宫内膜间质肉瘤HELQ和RAD51C蛋白的表达及其临床意义

刘 鹰，张 瑜，田 焱   

  • 出版日期:2020-07-20 发布日期:2020-07-20
  • 基金资助:

Expressions of HELQ and RAD51C in endometrial stromal sarcoma and their clinical significance

  

  • Online:2020-07-20 Published:2020-07-20

HTML

PDF

318

摘要： 目的 观察正常子宫内膜和子宫内膜间质肉瘤（ESS）组织POLQ样蛋白解旋酶（HELQ）和RAD51C蛋白表达情况，并分析其与ESS患者临床特征的相关性。方法 收集2013年1月~2016年12月在湖南省肿瘤医院接受手术治疗并确诊的ESS患者肿瘤组织（37例）及正常内膜组织标本（14例），采用免疫组织化学染色法检测组织中HELQ和RAD51C蛋白表达，分析其与患者年龄、FIGO分期、肿瘤大小和淋巴结转移的相关性。结果 免疫组织化学染色结果显示：ESS患者HELQ和RAD51C表达较正常组显著降低，并且HELQ和RAD51C表达呈正相关（P<0.05）。ESS中HELQ表达强度与肿瘤大小相关（P<0.05）。HELQ和 RAD51C表达强度与患者年龄、FIGO分期和淋巴结转移无关（P>0.05）。结论 提示HELQ与RAD51C参与的DNA同源重组修复可能与ESS的发生发展有关。

关键词: 子宫内膜间质肉瘤, HELQ, RAD51C, DNA损伤修复

Abstract: Objective To observe the expression of HELQ and RAD51C in normal endometrial and endometrial stromal sarcoma (ESS) and analyze their correlation with the clinical features of the patients. Methods The expressions of HELQ and RAD51C proteins were detected by immunohistochemical staining in normal endometrial tissues (14 cases) and tumor tissues from patients with ESS (37 cases) treated in Hunan Provincial Cancer Hospital from January, 2013 to December, 2016. The correlations of the expressions of the two proteins with the patients’age, FIGO staging, tissue type, tumor size, and lymph node metastasis were analyzed. Results Immunohistochemical staining showed that the expressions of HELQ and RAD51C were both decreased in ESS patients compared with the normal group, and there was a positive correlation between HELQ and RAD51C expression (P<0.05). HELQ expression in ESS was correlated with the tumor size and type. The expressions of HELQ and RAD51C were not correlated with the patients’ age, FIGO stage and status of lymph node metastasis (P>0.05). Conclusion Homologous recombination- directed DNA repair involving HELQ and RAD51C may participate in the occurrence and progression of ESS.

Key words: endometrial stromal sarcoma, HELQ, RAD51C, DNA damage repair