南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (05): 708-712.doi: 10.12122/j.issn.1673-4254.2020.05.15

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痰瘀同治法对糖尿病大鼠肾脏TGF-β1/Smad3信号通路的影响

储全根，蔡正银，储 俊，轩 云，程 婕，王 盼   

  • 出版日期:2020-05-20 发布日期:2020-05-20
  • 基金资助:

Intervention of phlegm and blood stasis inhibits TGF-β1/Smad3 signaling pathway in the kidney of diabetic rats

  

  • Online:2020-05-20 Published:2020-05-20

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摘要： 目的 观察痰瘀同治法通过调节TGF-β1/Smad3信号通路对糖尿病大鼠肾脏病变的影响。方法 选取SD大鼠随机分为：空白组（NC）、模型组（MC）、痰瘀同治组（RPDBS）、化痰组（RP）、化瘀组（DBS），予以单次注射链脲佐菌素溶液制备糖尿病模型，喂养4周后，RPDBS组、RP组和DBS组分别予以相应的药物干预8周，麻醉状态下取材。采用HE染色法观察肾脏组织病理学变化；Western blot法和实时荧光定量PCR法检测肾脏组织转化生长因子-β1（TGF-β1）及其信号蛋白Smad3蛋白和基因表达水平。结果 与MC组比较，光镜下各治疗组肾小球和肾小管结构和排列改善明显；各治疗组TGF-β1、Smad3和p-Smad3蛋白和基因表达水平均明显下调（P<0.05）；其中RPDBS组TGF-β1、Smad3和p-Smad3蛋白和基因表达水平较RP组和DBS组下调更明显（P<0.05）。结论 痰瘀同治可能通过抑制 TGF-β1/Smad3信号通路激活，延缓糖尿病肾病纤维化，发挥肾脏保护作用。

Abstract: Objective To observe the effect of traditional Chinese medicine for intervention of phlegm and blood stasis in regulating TGF-β1/Smad3 signaling and relieving nephropathy in diabetic rats. Methods SD rats were divided into blank group (NC), diabetic model group (MC group), intervention of phlegm and blood stasis (RPDBS) group, phlegm-removing (RP) group and blood-removing (DBS) group. Diabetic models were established in all the rats except for those in the blank group. After 4 weeks of feeding, the rats in RPDBS group, RP group and DBS group were given corresponding drug intervention for 8 weeks. HE staining was used to observe the changes in renal histopathology. Western blotting and real-time fluorescence quantitative PCR were used to detect the expression levels of transforming growth factor-β1 (TGF-β1) and Smad3. Results The structure and arrangement of the glomeruli and renal tubules improved significantly in the treatment groups in comparison with those in the MC group. The expression levels of TGF-β1, Smad3 and p-Smad3 were significantly downregulated at both the protein and mRNA levels in the treatment groups (P<0.05), and the down-regulation was more obvious in RPDBS group than in RP group and DBS group (P<0.05). Conclusion Intervention of phlegm and blood stasis may inhibit the activation of TGF-β1/Smad3 signaling pathway and delay diabetic nephropathy and fibrosis to protect the renal function in diabetic rats.