南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (01): 73-78.doi: 10.12122/j.issn.1673-4254.2020.01.18

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染色体多态性是体外受精胚胎多核形成的重要相关因素

陈志恒，杨 丽，易翠卿，刘 俊，孙 玲   

  • 出版日期:2020-02-19 发布日期:2020-01-20
  • 基金资助:

Chromosomal polymorphisms are associated with blastomere multinucleation in IVF/ICSI cycles

  

  • Online:2020-02-19 Published:2020-01-20

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摘要： 目的 探讨不孕夫妇不同类型染色体多态性与胚胎多核形成的关系。方法 采用回顾性病例对照研究分析1145对首次接受IVF/ICSI周期的不孕夫妇，术前常规进行染色体核型筛查，根据染色体分成2大组：正常对照组及染色体多态组；染色体多态组再分为染色体臂间倒位亚组、D/G组染色体多态亚组及（1，9，16）qh+多态亚组。比较各组卵裂期胚胎的多核率、临床妊娠率及活产率的差异。结果 1145对夫妇中有139对夫妇（6.10%）至少一方存在染色体多态性，不同染色体多态组与正常对照组在女方年龄、体质量指数、基础促卵泡生成素水平、平均促性腺激素剂量、扳机日雌激素水平、平均获卵数、受精率、优胚率、临床妊 娠率和活产率上均无显著性差异（P>0.05），不同组胚胎多核率分别为：臂间倒位亚组8.23%，D/G组染色体多态亚组4.65%，较正常对照组（2.69%）显著增高（P<0.05），（1，9，16）qh+多态组胚胎多核率（2.77%）与对照组（2.69%）比较无显著差异（P>0.05）。结论 不孕夫妇染色体中臂间倒位和D/G组染色体多态性会增加胚胎多核率（1，9，16）qh+多态对胚胎多核率无影响。

Abstract: Objective To investigate the association of chromosomal polymorphisms with multinucleated embryos in infertile couples undergoing in vitro fertilization-intracytoplasmic sperm injection (IVF-ICSI). Methods This retrospective case-control study was conducted among 1145 infertile couples undergoing their first IVF/ICSI cycles. According to their karyotype, the couples were divided into chromosomal polymorphism group and control group, and the former group was divided into 3 subgroups: inversion group, D and G genome polymorphic group and 1, 9, and 16 qh+group. The blastomere multinucleation rate, clinical pregnancy rate and live birth rate were compared between the groups. Results Of the total of 1145 couples, 139 (6.10% ) had chromosomal polymorphisms at least in one partner. No significant differences were found in female age, BMI, basal FSH level, total gonadotropin dose, E2 level on day of HCG, number of oocytes retrieved, fertilization rate, top quality embryo rate, clinical pregnancy rate or live birth rate among the groups (P>0.05). The multinuclear rate of the embryos in couples with pericentric inversion of chromosomes 1, 9, and Y chromosomes and those with D and G genome polymorphisms were 8.23% and 4.65%, respectively, significantly higher than that in the control group (2.69%; P<0.05); the multinuclear rate of the embryos was 2.77% in 1, 9, and 16 qh+ group, similar with that in the control group (P>0.05). Conclusion Infertile couples with pericentric inversion of chromosomes 1, 9, and Y chromosomes and in those with D and G genome polymorphism are at higher risks of blastomere multinucleation in IVF- ICSI cycles; 1, 9, and 16 qh + polymorphisms do not increase the rate of blastomere multinucleation of the embryos.