南方医科大学学报

南方医科大学学报 ›› 2019, Vol. 39 ›› Issue (12): 1482-1486.doi: 10.12122/j.issn.1673-4254.2019.12.13

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可溶性晚期糖基化终产物受体：在非PCOS患者卵泡液中的水 平与其促排后卵巢反应性相关

李美玲，卲 永，伏海燕，周 城，姚 兵，谈 勇   

  • 出版日期:2019-12-27 发布日期:2019-12-20
  • 基金资助:

Correlation between follicular fluid sRAGE level and ovarian responsiveness in non-PCOS patients undergoing controlled ovarian hyperstimulation

  

  • Online:2019-12-27 Published:2019-12-20

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摘要： 目的 探讨非PCOS卵泡液可溶性晚期糖基化终产物受体（sRAGE）水平与其促排后卵巢反应性的相关性。方法 ELISA法测定90例非PCOS患者常规长方案COH患者取卵日卵泡液中sRAGE浓度；收集患者临床基础状态、激素水平、获卵数以及受精率等数据。采用SAS统计软件对各项指标进行分析。结果 卵泡液的sRAGE水平与基础FSH（P=0.0036）、Gn使用量（P< 0.0001）呈显著负相关关系。卵泡液的sRAGE水平与AFC（P<0.0001）、获卵数（P<0.0001），受精率（P=0.0047）呈显著正相关关系。并按照获卵数分为3组：目标获卵数以下（<7个）、在目标标获卵数范围内（7-15），在目标获卵数目以上（>15个）；单因素方差分析卵泡液sRAGE水平与获卵数呈正相关关系，与明显高于目标获卵数组和目标获卵数目以下组（P值分别为：P<0.0001 和 P=0.0012）。结论 卵泡液中sRAGE水平与非PCOS患者卵巢储备功能相关，卵泡液中sRAGE水平可以反映COH后获卵数与受精率情况，可以预测在控制性超促排卵过程的卵巢反应性。

Abstract: Objective To investigate the correlation between soluble receptor for advanced glycation end products (sRAGE) level in the follicular fluid and ovarian responsiveness in non-PCOS patients undergoing controlled ovarian hyperstimulation. Methods Ninety non-PCOS patients underwent IVF/ICSI using a short-acting long protocol for ovarian stimulation with a GnRH agonist. For each patient, the level of sRAGE in the follicular fluid was measured by enzyme linked immunosorbent assay (ELISA), and the data including the clinical baseline state, hormone level, number of oocytes obtained and the fertilization rate were collected. Results Follicular fluid sRAGE level showed significant negative correlations with basal FSH level (P=0.0036) and Gn dose (P<0.0001) and positive correlations with AFC (P<0.0001), number of oocytes obtained (P<0.0001), and the fertilization rate (P=0.0047). Follicular fluid sRAGE level was positively correlated with the number of oocytes obtained, and was significantly higher in cases with oocytes obtained above the target number (>15) than in cases with oocytes obtained within the range of the target numbers (7-15) and below the target number (<7) (P<0.0001 and P=0.0012, respectively). Conclusion Follicular fluid sRAGE level can reflect ovarian reserve function in non-PCOS patients, the number of oocytes obtained and the fertilization rate, and can thus predict ovarian responsiveness during controlled hyperstimulation in nonPCOS patients.