南方医科大学学报

南方医科大学学报 ›› 2019, Vol. 39 ›› Issue (09): 1078-.doi: 10.12122/j.issn.1673-4254.2019.09.12

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华蟾素对骨癌痛大鼠背根神经节细胞瞬时外向钾通道电流的影响

朱时钰，刘丹，胡卫，杨红卫   

  • 出版日期:2019-09-20 发布日期:2019-09-20

Effect of cinobufagin on transient outward potassium current in dorsal root ganglion cells of rats with cancer-induced bone pain

  • Online:2019-09-20 Published:2019-09-20

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摘要： 目的观察华蟾素对骨癌痛（CIBP）大鼠背根神经节细胞瞬时外向钾电流（IA）的影响，探讨华蟾素可能的镇痛机制。方法 急性分离SD大鼠背根神经节（DRG）细胞，应用全细胞膜片钳技术记录华蟾素对骨癌痛大鼠DRG细胞IA的影响。结果骨癌痛 大鼠背根神经节细胞IA电流密度减小，激活曲线向右移动，失活曲线向左移动；华蟾素能调节骨癌痛大鼠背根神经节细胞IA电 流密度，并且逆转骨癌痛IA电流激活和失活曲线的改变。结论在大鼠胫骨内注入肿瘤细胞后，DRG神经元IA电流减小；华蟾素 可能通过调节大鼠背根神经节细胞IA电流激活和失活特性，该作用可能与它的镇痛机制有关。

Abstract: Objective To observe the effect of cinobufagin on transient outward potassium current (IA) in rat dorsal root ganglion cells of cancer-induced bone pain (CIBP) and explore the possible analgesic mechanism of cinobufagin. Methods Whole cell patch clamp technique was used to examine the effect of cionbufagin on IA in acutely isolated dorsal root ganglion (DRG) cells from normal SD rats and rats with bone cancer pain. Results The DRG cells from rats with CIBP showed obviously decreased IA current density, an activation curve shift to the right, and an inactivation curve shift to the left. Cinobufagin treatment significantly increased the IA current density and reversed the changes in the activation and inactivation curves in the DRG cells. Conclusion IA current is decreased in DRG neurons from rats with CIBP. Cinobufagin can regulate the activation and inactivation of IA current in the DRG cells, which may be related to its analgesic mechanism.