南方医科大学学报

南方医科大学学报 ›› 2019, Vol. 39 ›› Issue (07): 855-.doi: 10.12122/j.issn.1673-4254.2019.07.17

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益气养阴活血中药对糖尿病肾病大鼠Notch/Hes1 通路与血管内皮CD34、CD144 的影响

周雪梅，徐从书，王凯，储全根，董昌武，吴传云，赵建根，李玲秀，汪莉   

  • 出版日期:2019-07-20 发布日期:2019-07-20

Effect of traditional Chinese medicine for replenishing qi, nourishing yin and activating blood on renal Notch/Hes1 signaling in rats with diabetic nephropathy

  • Online:2019-07-20 Published:2019-07-20

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摘要： 目的观察益气养阴活血中药对糖尿病肾病大鼠Notch/Hes1通路与血管内皮CD34、CD144作用机制。方法采取左肾切 除术+高脂高糖饲料喂养联合腹腔注射STZ复制早期糖尿病肾病大鼠模型，将建模成功的大鼠随机分为模型组、西药贝拉普利 组、中药高剂量组、中药中剂量组、中药低剂量组，设正常对照组，药物干预8周。观察24 h尿白蛋白、HE染色肾组织病理、免疫 组化肾组织Hes1、Western blot法检测CD34、CD144的表达。结果与正常组比较，模型组24 h尿白蛋白、Hes1、CD34、CD144的 含量比较均有明显差异（P<0.05）；与模型组比较，中药高剂量组、中剂量组能降低24 h尿蛋白（P<0.05），中药各剂量组均能明显 降低肾组织Hes1、CD34含量（P<0.05），中药高剂量组均能明显降低肾组织CD144含量（P<0.05）；与西药组比较，中药各剂量组 均能降低CD34（P<0.05），中药低剂量组降低CD144（P<0.05）。病理观察模型组大鼠出现肾小球硬化、萎缩，系膜基质增多、系 膜出现增厚增宽并可见结节样增生等改变；中药高、中剂量组对上述病理改变有积极改善。结论益气养阴活血中药可以降低 模型大鼠肾组织Hes1和CD34、CD144，发挥对肾功能的保护作用，延缓DN的发展。

Abstract: Objective To observe the effects of a traditional Chinese medicine (TCM) capsule for replenishing qi, nourishing yin and activating blood on Notch/Hes1 signaling pathway in the renal tissue and vascular endothelial CD34 and CD144 expressions in a rat model of diabetic nephropathy. Methods Rat models of early-stage diabetic nephropathy were established by left nephrectomy and high- fat and high- sugar feeding combined with intraperitoneal injection of STZ. The rats were randomized into model group, benazepril group, and high-, moderate-, and low-dose TCM capsule groups for corresponding treatments, with 6 normal rats as the control group. After 8 weeks of drug treatment, blood glucose and 24-h urinary albumin of the rats were measured, and the renal histopathology was observed with HE staining; Hes1 expression in the renal tissue was detected with immunohistochemical staining, and the renal expressions of CD34 and CD144 were detected using Western blotting. Results Compared with the normal control group, the rat models of diabetic nephropathy showed obvious abnormalities in 24-h urinary albumin and expressions of Hes1, CD34 and CD144d. The TCM capsule at both the high and moderate doses significantly reduced 24-h urinary albumin in the rats; the renal expressions of Hes1 and CD34 was significantly reduced in all the dose groups, and the expression of CD144 was significantly reduced in the high-dose group. Compared with benazepril group, the TCM capsule obviously reduced CD34 expression at all the 3 doses and lowered CD144 expression at the low dose. Histopathologically, the rats in the model group showed glomerular hypertrophy, increased mesenteric matrix, thickening and widening of the mesenteric membrane, and nodular hyperplasia. These pathologies were obviously alleviated by treatment with the TCM capsule at the high and moderate doses. Conclusion The Traditional Chinese medicine (TCM) capsule for replenishing qi, nourishing yin and activating blood can reduce Hes1, CD34 and CD144 in kidney tissue of model rats, play a protective role on kidney function and delay the development of DN.