南方医科大学学报

南方医科大学学报 ›› 2019, Vol. 39 ›› Issue (06): 724-.doi: 10.12122/j.issn.1673-4254.2019.06.15

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健康人群CDKN2A和CDKN2B基因甲基化水平与衰老的相关性

郑中华，季慧慧，陈楚嘉，李银，段世伟   

  • 出版日期:2019-06-20 发布日期:2019-06-20

Correlation between methylation level of CDKN2A and CDKN2B genes and aging in healthy individuals

  • Online:2019-06-20 Published:2019-06-20

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摘要： 目的在正常人群中寻找细胞周期蛋白依赖性激酶抑制因子（CDKN）2A、CDKN2B基因甲基化水平与衰老之间的关系。 方法我们收集了284名男性和246名女性体检者的外周血，采用甲基化特异性实时定量聚合酶链技术（qMSP）检测CDKN2A、 CDKN2B基因甲基化水平。使用Pearson直线相关系数或Spearman秩相关系数来分析CDKN2A和CDKN2B基因甲基化水平 与衰老的关系。结果两个基因甲基化水平之间呈正相关（P<0.05）。在总体样本组和女性组中，均发现CDKN2A基因甲基化 水平与年龄呈负相关（P<0.05）。在总体样本组中，CDKN2A、CDKN2B基因甲基化水平与甘油三酯（TG）、载脂蛋白E（ApoE）、 脂蛋白（a）[Lp(a)]、谷草转氨酶（AST）均呈负相关（P均<0.05）。性别分层分析后发现，女性组和男性组中两个基因甲基化水平 均与Lp(a)呈负相关（P<0.05）。男性组中CDKN2A基因甲基化水平与AST呈负相关（P<0.05），而CDKN2B基因甲基化水平与 高密度脂蛋白（HDL）、ApoE 呈负相关（P 均<0.05）。女性组中CDKN2A 基因甲基化水平与低密度脂蛋白（LDL）呈正相关， ApoE、AST呈负相关（P均<0.05）。结论CDKN2A、CDKN2B基因甲基化水平与年龄以及多种蛋白指标密切相关。

Abstract: Objective To analyze the relationship between CDKN2A and CDKN2B gene methylation with aging in the general population. Methods We collected peripheral blood samples from 284 male and 246 female healthy subjects for detection of methylation levels of CDKN2A and CDKN2B genes using quantitative methylation-specific PCR (qMSP). The relationship between the methylation levels of CDKN2A and CDKN2B genes and aging was analyzed using Spearman or Pearson correlation test. Results We found a significant positive correlation between the methylation levels of the two genes in these subjects (P<0.05). In the overall population as well in the female subjects, CDKN2A methylation was found to be inversely correlated with age (P<0.05). The methylation levels of CDKN2A and CDKN2B genes were inversely correlated with TG, ApoE, Lp(a) and AST in the overall population (P<0.05). In both the female and male subjects, the methylation levels of the two genes were inversely correlated with Lp(a) (P<0.05). In the male subjects, CDKN2A methylation was inversely correlated with AST (P< 0.05), while CDKN2B methylation was inversely correlated with HDL and ApoE (P<0.05). In the female subjects, CDKN2A methylation was positively correlated with LDL and inversely correlated with ApoE and AST (P<0.05). Conclusion The methylation levels of CDKN2A and CDKN2B are closely related to age and the levels of multiple proteins in healthy subjects.