南方医科大学学报

南方医科大学学报 ›› 2019, Vol. 39 ›› Issue (04): 387-.doi: 10.12122/j.issn.1673-4254.2019.04.02

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PSMA7在胃癌中的表达及对胃癌增殖、侵袭迁移及成瘤能力的影响

夏淑晶，张莉莉，成凤干，冯振卿，陆伦根   

  • 出版日期:2019-04-20 发布日期:2019-04-20

Expression of PSMA 7 and its effect on proliferation, invasion, migration and tumorigenesis of gastric cancer

  • Online:2019-04-20 Published:2019-04-20

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摘要： 目的研究PSMA7在胃癌中的表达及其对胃癌增殖、侵袭迁移及裸鼠皮下成瘤能力的影响。方法收集60例胃癌患者的 癌组织及配对的癌旁组织，应用免疫组化法检测胃癌和配对癌旁组织中PSMA7的表达水平；应用慢病毒转染技术在胃癌细胞 株SGC7901中干扰PSMA7；应用Cell Counting Kit-8（CCK-8）和细胞克隆形成实验检测胃癌细胞增殖能力的变化，Transwell实 验检测胃癌细胞侵袭迁移能力的变化；稳转细胞株构建裸鼠皮下移植瘤模型，研究干扰PSMA7对皮下移植瘤的影响。结果 免疫组化结果显示PSMA7在胃癌组织中的表达水平显著高于其配对的癌旁组织（P<0.05）；在人胃癌细胞株SGC7901中下调 PSMA7表达能够显著抑制肿瘤细胞的增殖、侵袭迁移能力（P<0.05）；在BALB/c小鼠皮下移植瘤模型中干扰PSMA7的表达能 够明显抑制皮下移植瘤的生长（P<0.05）。结论PSMA7在胃癌组织中高表达，干扰PSMA7抑制胃癌细胞增殖、侵袭迁移及裸 鼠皮下成瘤的能力。

Abstract: Objective To study the expression of PSMA7 and its effect on proliferation, invasion and migration of gastric cancer and subcutaneous tumorigenesis in nude mice. Methods Specimens of tumor tissues and paired adjacent tissues were collected from 60 patients with gastric cancer for detecting the expression levels of PSMA7 using immunohistochemical method. Gastric cancer cell line SGC7901 was transfected with a lentiviral vector to inhibit PSMA7 expression, and the changes in cell proliferation and invasion were observed using cell counting kit-8 (CCK-8), clone formation assay and Transwell assay. A BALB/c mouse model bearing subcutaneous gastric cancer xenograft was established using SGC7901 cells with stable PSMA7 knockdown to assess the effect of low expression of PSMA7 on xenograft growth. Results Gastric cancer tissues expressed significantly higher levels of PSMA7 than the paired adjacent tissues (P<0.05). In SGC7901 cells, interference of PSMA7 expression significantly inhibited the cell proliferation and invasion (P<0.05). In the tumor-bearing BALB/c mice, the xenografts derived from SGC7901 cells with PSMA7 expression interference showed significant growth suppression as compared with the control xenografts (P<0.05). Conclusion PPSMA 7 is overexpressed in gastric cancer tissues, and PSMA7 knockdown inhibits the proliferation, invasion, migration and subcutaneous tumorigenesis of gastric cancer cells in nude mice.