南方医科大学学报

南方医科大学学报 ›› 2018, Vol. 38 ›› Issue (10): 1239-.doi: 10.12122/j.issn.1673-4254.2018.10.14

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高表达MMP20可能是子宫内膜癌患者预后不良的潜在预测因子

赵倩，刘蜀   

  • 出版日期:2018-10-20 发布日期:2018-10-20

High expression of MMP20 may be a potential prognostic factor for poor outcomes of patients with endometrial carcinoma

  • Online:2018-10-20 Published:2018-10-20

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摘要： 目的探讨MMP20表达与子宫内膜癌发病临床参数及预后之间的相关性。方法生物信息学分析MMP20在子宫内膜癌 中的表达与预后之间的关系。进一步收集21例配对子宫内膜癌与癌旁新鲜组织，提取RNA，实时荧光定量PCR检测MMP20 在这些组织中的表达水平。此外，收集134例子宫内膜癌组织和34例子宫内膜组织，免疫组化检测MMP20的蛋白表达，统计分 析MMP20 蛋白表达变化与子宫内膜癌临床参数及预后之间的关系。结果生物信息学TACG数据库分析发现，与MMP20 mRNA低表达患者相比，MMP20 表达增高的子宫内膜癌患者生存时间显著缩短（P<0.05）。实时荧光定量PCR检测发现， MMP20 mRNA在子宫内膜癌组织中的表达显著高于配对子宫内膜组织（P<0.05）。免疫组化检测显示，MMP20蛋白在子宫内 膜癌组织中的表达亦显著高于子宫内膜组织（P<0.05），且MMP20蛋白表达的增加与子宫内膜癌患者的FIGO分期呈显著正相 关（P<0.05），而与患者的生存时间呈显著负相关（P<0.05）。多变量分析显示，MMP20表达是子宫内膜癌患者预后的潜在独立 预测因子（P>0.05）。结论MMP20蛋白高表达是子宫内膜癌预后不良的潜在预测因子。

Abstract: Objective To explore the expression of MMP20 in endometrial carcinoma and its clinical significance. Methods Bioinformatics analysis was used to explore the correlation of MMP20 expression with the prognosis of the patients with endometrial carcinoma. We collected 21 pairs of fresh endometrial carcinoma and adjacent endometrial tissues to detect the expression of MMP20 mRNA using real-time PCR. We also examined MMP20 protein expressions in 134 paraffin-embedded endometrial carcinoma tissues and 34 paraffin-embedded endometrial tissues using immunohistochemistry. The correlation of MMP20 expression with the clinicopathological features and prognosis of the patients were analyzed. Results Based on TCGA database analysis, the patients with endometrial carcinoma showing an increased MMP20 mRNA expression had a significantly poorer prognosis than those with a low MMP20 mRNA expression (P=0.00065). Real-time PCR analysis and immunohistochemistry confirmed the up-regulated expressions of MMP20 at both the mRNA (P=0.0062) and protein (P=0.005) levels in endometrial carcinoma tissues compared to the adjacent endometrial tissues. An elevated MMP20 protein expression was positively associated with FIGO stage of endometrial carcinoma (P=0.014) and inversely correlated with the survival time of the patients (P=0.019). The Cox regression model analysis showed that an increased MMP20 expression was an independent predictor of a poor prognosis of the patients with endometrial carcinoma (P=0.067). Conclusion High expression of MMP20 is a potential prognostic factor for poor outcomes of patients with endometrial carcinoma.