To investigate the surgical treatment of cervical intraspinal tumor and the influence of instrumentation.

A total of 27 patients with cervical intraspinal tumor surgically treated from January 2011 to December 2014 were retrospectively analyzed. Patients were divided into the internal fixation group (13 cases) and the simple tumor resection group (14 cases). No significant differences were found in gender, age, disease duration between 2 groups (P>0.05). JOA score was analyzed before and after operation, the cervical spine radiographs and MR images were recorded.

All tumors in the 27 patients were primarily removed. The follow-up time ranged from 1 to 56 months with an average of 29.7 months. Radicular pain, sensory disturbances, movement disorders and altered reflexes were significantly improved, JOA score increased from 11.7 points preoperatively to 14.9 points at the end of the follow-up (P < 0.05). No significant difference was found in JOA score improvement rate between 2groups (P>0.05). In the simple tumor resection group, 2 cases appeared recurvation deformity in cervical spine.

Surgical treatment can achieve good clinical efficacy in the treatment of cervical intraspinal tumor with or without fixation.

To assess the clinical efficacy of laparoscopic partial bladder resection in the treatment of bladder diverticulum and to explore the application value of it.

Retrospective analysis was applied to analysis cases who were given laparoscopic partial bladder resection from January 2014 to October 2015. Four cases were given transurethral enucleativeresection of the prostate and laparoscopic partial bladder resection, 1 case was underwent transurethral bladder neck incision surgery and laparoscopic partial bladder resection.

The surgeries were successful in 5 cases. The average operation time was 140±23.7 min, with average bleeding volume of 65±15.2 mL. There was no need of blood transfusion. There were no serious complications after the operation. The drainage tube was removed 3 d later after operation. The average hospitalization time was 7.4±1.1 d.

For bladder outlet obstruction (BOO) cases complicated with bladder diverticulum, transurethral enucleativeresection of the prostate (TUERP) and laparoscopic partial bladder resection showed the advantages of less trauma, less bleeding, quick recovery, fewer complications, less hospitalization. microinvasive treatment of bladder diverticulum is a safe and feasible operation method.

To summarize the CT and MRI features of posterior mediastinal ganglioneuroma.

Imaging data (CT or MRI) of 18 posterior mediastinal ganglioneuroma patients were retrospectively reviewed, and the imaging features were summarized in comparison with pathology.

Eleven cases under 20 years of age were occurred, with 12 female. The lesion size were large, the largest diameter of 12 cases were greater than 3 cm. CT value was about 20-40 Hu, and most tumors were homogeneous in density with low signal intensity on T1WI, high signal intensity on T2WI.Two cases of tumors showed low signal with no enhancement at early stage and slight enhancement in delayed phase.

The posterior mediastinal ganglioneuroma has certain imaging features, and it can be diagnosed accurately by imaging method.

To investigate the relationship between pulmonary focal ground-glass opacity (fGGO) and bronchial and the correlation with the pathological types by MSCT.

A total of 86 patients and 89 lesions who had≤3.0 cm fGGO that detected on 256 iCT scan were enrolled in this study, including 29 benign lesions, 11 lesions were preinvasive lesions and 49 lesions of pulmonary adenocarcinoma.The solid component proportion of fGGO and its relationship with bronchiole were determined on the cross sectional plane、multiple plane reconstruction (MPR)、curve planar reformation (CPR)、MinIP images According to the proportion of solid component which was measured on CT images, fGGO lesions were divided into class A (pure GGO), class B (51%-99% ground glass) and class C (1%-50% ground glass).The correlation of fGGO with bronchiole were divided into 5 types: type Ⅰwith the bronchiole obstructed; type Ⅱ with the bronchiole of tortuous and dilated fGGO shadow; type Ⅲ within ground glass area the bronchiole was tortuous and dilated; type Ⅳ with normal bronchial course and ground glass area; type Ⅴ with the bronchiole running its way by the side of the lesion and did not enter fGGO.

(1) The presence rate of certain relationship between GGO and bronchiole in the three groups were 65.5%(19/29)、36.4% (4/11) and 95.9% (47/49), respectively, with significant differences (χ2=26.758, P < 0.001). (2) In type Ⅰ, the lesion number of the benign lesion group, preinvasive group and invasive group were 0, 0 and 16 respectively, which was 2, 0 and 15 respectively in type Ⅱ, 2.0 and 6 respectively in type Ⅲ, 9, 2 and 5 respectively in type Ⅳ, and 6, 2 and 5 respectively in type Ⅴ.The differences were significant (P < 0.001).In benign lesion group type Ⅳ and Ⅴ were frequent seen, in pre-invasive lung cancer group, the lesions usually showed no certain relations with the bronchi, while in infiltrative adenocarcinomas type Ⅰ and Ⅱ were commonly found. (3) The lesion number of class A, B and C were 13, 32 and 44 respectively. A positive correlation existed between the proportion of ground glass and the bronchiole-related type (r=0.442, P < 0.000). The different nature of fGGO were different from the type of bronchus with significant differences.

MSCT scanning and multiple reconstruction Methods can clearly show the correlation of fGGO with bronchi and the morphological characteristics. It can infer the organization type with vital significance to the clinical treatment.

To evaluate the high frequency color Doppler ultrasound features of thyroid microcarcinoma, and explore the value of ultrasound in the diagnosis of thyroid microcarcinoma.

We retrospectively analyzed ultrasound findings of 60 malignant nodules in 42 cases of small thyroid cancer confirmed by surgery and pathology, including the internal echo of the nodules, edge feature, aspect ratio, calcification size, the distribution of blood flow signal. It was compared with 48 cases benign nodules (diameter≤1).

There were significant differences in terms of low internal echo, fuzzy edge, micro calcification, the aspect ratio≥1, blood flow signal distribution aspect (P < 0.05).

The high frequency color Doppler ultrasound has important clinical value in the diagnosis of thyroid microcarcinoma.

To explore the clinical efficacy of the double Endobutton plate and Clavicular Hook Plate fixation treatment for Tossy Ⅲ acromioclavicular joint dislocation.

From June 2011 to June 2016, 40 patients of Tossy Ⅲ acromioclavicular joint dislocation admitted to the wuxi xishan hospital were enrolled, including 12 males and 8 females, aged from 21 to 55 years old with an average age of 33.The time from injury to operation was 1-4 days, averaged 2 days, the application of antibiotics was no more than 3 days after operation. They were divided into two groups according to the different methods of operation: double endobutton plate group with 20 cases and clavicular hook plate group with 20 cases.

Compared with clavicular hook plate group, double endobutton plate group had significant difference in incision, operation time, intraoperative blood loss, shoulder 's function recovery (P < 0.05).All patients'incisions were healed well, with no early complications such as blood vessels and nerves'injury and postoperative infection. The patients were followed up from 3 to 24 months, there was no internal fixation loosening and joint dislocation were occured during the follow-up period.

Double Endobutton technique is an effective method on treatment of Tossy Ⅲ type acromioclavicular joint dislocation with minimal invasive, simple operation and satisfied clinical effect.It is suitable for basic-level hospital.

To explore the value of transvaginal ultrasound in the diagnosis of polycystic ovary syndrome.

A total of 60 patients with polycystic ovary syndrome (experimental group) and 60 cases of the same period to our line of normal women of childbearing age (control group) were enrolled as research objects from January 2012 to December 2012. Two groups were both performed with vaginal ultrasound examination, and patients in the experimental group also were performed with abdominal ultrasonography. Number of follicles, ovarian volume, pulsatility index and the resistance index were cmpared in two groups and two checking modes.

There were significant differences in the number of follicles, ovarian volume, pulsation index and resistance index between the two groups, the results of the two groups were significantly different (P < 0.05).

Vaginal ultrasound can display ovarian features more intuitive and clear, it can improve the level of diagnosis accuracy.

To investigate the imaging diagnosis of bone marrow edema syndrome of the hip.

The imaging data of 13 cases (9 males and 4 females) of bone marrow edema syndrome were collected. All patients were followed-up for 3 to 6 months.13 patients underwent MRI scanning and 5 patients underwent X-ray examination.

Thirteen cases of bone marrow edema syndrome of the hip, right hip in 8 cases, left hip in 5 cases. The bone marrow edema pattern involved the femoral head and femoral neck in 10 hips. The bone marrow edema involved thefemoral head and femoral neck and femoral trochanteric in 3 cases. The MRimages demonstrated low signal intensity in all 13 cases on T1WI and normal signal intensityin 1 case on T2WI.moderateor high signal intensity in 12 cases on T2WI and high signal on T2 fat suppressed in all 13 cases. A small joint effusion was observed in 4 cases on T2WI. X-ray manifestations: femoral head and femoral neck bone density decrease in 5 cases. The femoral head shape and bone cortex were normal in all 13 cases.

Familiar with the imaging findings of bone marrow edema syndrome of the hip canimprove the correct diagnosis.

To explore the diagnostic value of pulmonary artery pressure value combined with right lower pulmonary artery diameter in pulmonary heart disease.

Randomly selected from June 2014 to May 2016, 49 cases with pulmonary heart disease diagnosed as pulmonary heart disease group; 31 cases with chronic obstructive pulmonary disease were treated with the same time as the COPD group; and 30 cases of normal adults as control group. CT was used to measure the diameter of the right lower pulmonary artery in each group, and the pulmonary artery pressure was measured by echocardiography. The indexes of each group were compared and the relationship between the indexes and the course of pulmonary heart disease were analyzed.

With the development of pulmonary heart disease, the diameter of the right pulmonary artery increased, there were significant differences in the diameter of the main pulmonary artery and pulmonary artery pressure between the control group and the pulmonary heart disease group. Pulmonary heart disease group and COPD group, the control group of the right lower pulmonary diameter/pulmonary artery pressure value comparison, the difference was statistically significant.

The change of the right lower pulmonary diameter and pulmonary pressure value are related to the degree of the disease course of pulmonary heart disease. The value of right lower pulmonary diameter and pulmonary artery pressure value is a CT monitoring index in the diagnosis of pulmonary heart disease and its process.

To analyze the MSCT features of diffuse panbronchiolitis (DPB) and improve the diagnostic accuracy.

CT features of DPB in 13 patients were retrospectively analyzed.

Among the 13 cases, the distribution of lesions was bilateral with diffuse in 12 cases and local in 1 case. The chest CT apperances of DPB were as follows:1diffuse small nodules accompanied by tree-in-bud sign in 13 cases; 2small airway bron-Chiectasis in 12 cases, mucoid impaction in 3 cases; 3inflamma-tory patchy consolidation in 8 cases, small cavity in 2 cases, interstitiell fibrosis in 3 cases; 4 Chronic paranasal sinusitis was found by CT examination in 11 cases, mostly involving the maxillary sinus; the changes of CT features before and after treatment; the lesions were absorptive in different degree after treatment 1-3 months later.

MSCT features of DPB are characteristic, playing an important role in the diagnosis of DPB.

To To evaluate the combination of transperineal with endoanal high-frequency doppler ultrasonography for detecting the lesions of perianal infection disease.

Fifty-five patients who suffered from perianal infection disease were enrolled in the study, of which includes 49 cases were perianal abscess and 6 cases, fistula-in-ano. The patients underwent transperineal ultrasound examinations with high frequency and doppler probe and, sequentially via endoanal ultrasound. The anatomic location, the adajacent soft-tissue and the ultrasound features of the lesions were evaluated. The efficiency of ultrasound was analyzed according to the operation and clinical diagnosis.

Forty-eight cases of perianal abscess and 4 cases of fistula-in-ano were detected via the transperineal ultrasonography. Combined the endoanal ultrasonography, additional 4 cases of perianal abscess and 2 cases of fistula-in-ano could be revealed clearly. The combined ultrasonography could show the anatomic locations, classification of Parks, and the relationship with the adjacent soft tissues, as well as its ultrasonography features.

Combination of transperineal with endoanal ultrasonography with high frequency and doppler technique could well document the perianal infection disease, providing clearly lesion anatomic location and its adjacent soft tissues relationship, which plays an vital role for the treatment of the disease.

To explore the effect and mechanism of apoptosis stimulating protein2 of p53 (ASPP2) in osteosarcoma cells metastasis.

Quantitative-Polymerase Chain Reaction (Q-PCR) was performed to determine ASPP2 mRNA in eight samples of osteosarcoma tissue and four cell lines of osteosarcoma. The levels of ASPP2 protein were detected by western blot. The metastasis ability of SAOS2 cells that overexpressed ASPP2 were estimated by Transwell experiment. The Levels of interaction of β-catenin and E-cadherin were analyze by co-IP. Immunofluorescence was used to detect the expression of ASPP2 protein in the nuclear of osteosarcoma cells.

The ASPP2 mRNA levels in eight osteosarcoma tissue samples were decreased significantly than that in normal bone tissue. Compared with normal human osteoblast cells HOSB, the levels of BRMS1 mRNA and protein obviously decreased in osteosarcoma cell lines OS187、SAOS2、HOS and MG63. The metastasis ability, β-catenin-E-cadherin complex and β-catenin nuclear-translocation of SAOS2 cells was significantly inhibited after ASPP2 overexpression.

BRMS1 can inhibit SAOS2 cells metastasis and the mechanism may involve with stabilizing the β-catenin-E-cadherin complex, and suppress β-catenin nuclear-translocation.

To analyze the effect factors of acute radiation proctitis caused by intensity modulated radiotherapy for cervical cancer.

From march 2013 to may 2015, 80 cervical cancer patients were received intensity modulated radiotherapy and eligible for this study. In all the patients, 55 patients accepted operation, the other 25 patients did not accepted operation before radiation therapy. Clinical stage I of 30 cases, stage Ⅱ of 37 cases, stage III of 13 cases; 80 patients received treatment of intensity modulated radiotherapy and post irradiation. All of the patients received the biological effective dose (BED) between 45-50 Gy. If there was swollen lymph nodes, then given the synchronization to 60-70 Gy. Intracavitary radiotherapy with high dose rate afterloading machine, The dose of radiotherapy of the patients without surgery was 35-42 Gy/6-7 times, single 5-6 Gy, The dose of radiotherapy of the patients with surgery was 15 Gy/3 times, single 5 Gy. The correlation of every variable were evaluated by spearman relationship analysis. The rate of irradiated pneumonitis was analyzed by χ2 test, the multivariate effect on irradiated pneumonitis was statisticed by Logistic regression model.

In the 80 patients, rate of acute radiation proctitis was 57.5%, and the rate of 1 degree was 46.25%, the rate of 2 degree was 11.25%, no degree Ⅲ、IV acute radiation proctitis Univariate analysis showed rectal average dose、rectal V30、V35、V40、V50 were important effect factors for acute radiation proctitis. Father more, rectal V40 was likely to be the independent factors of acute radiation proctitis by logistic model.

Rectal V40 is the independent effect factor of acute radiation proctitis.

To observe the clinical effect and mechanisms of Banxia Houpu decoction for refractory gastroesophageal reflux disease (rGERD).

A total of 80 patients were randomly divided into two groups. The control group was given rabeprazole and mosapride. The experimental group was administered with Pinelliae and Magnoliae Officinalis Decoction besides the western medicine. Gerd Q and high resolution manometry were performed before and after 8 week treatment.

After treatment, the Gerd Q score of the experimental group was significantly improved compared with the control group. The total effective rate of experimental group was 94.74% while the control group was 77.14%. And the resting pressure of esophageal LES (15.01±7.79 mmHg) was significantly higher than that before treatment (14.35±7.83 mmHg) in the experimental group. The difference was statistically significant (P < 0.05).

Banxia Houpu decoction has significant effect in treatment of rGERD. It can improve the resting pressure of the esophageal LES.

To investigate the effect of radiofrequency ablation combined sequential nonspecific immune treatment on patient with hepatic carcinoma.

Before and after the radiofrequency ablation combined sequential nonspecific immune treatment, the percentage of CD4+ T cells, CD8+ T cells and NK cells on peripheral blood were evaluated by flow cytometry.

After the nonspecific immune treatment, the percentage of CD4 + T cells, NK cells and CD4+/CD8 + ratio were significantly higher than that without treatment and CD8+ was significantly decreased (P < 0.05).

Non-specific immune treatment can improve the immune function in HCC after RFA.

To compare convention dissection with coblation by low-temperature plasma radiofrequency in tonsillectomy intra operation and after operation.

Eighty patients were divided into convention dissection group and low-temperature group, each group was divided into two groups by age. Operation time, bleeding volume, postoperative pain time were recorded, and the differences among these 4 groups were analyzed.

Coblation groups had a significant shorter operative time, less bleeding intraoperative, and a better pain sore after operation than the traditional method groups. At the same time, the teenages had a better effect than the elders in coblation group.

Comparing with traditional dissection, coblation by low-temperature plasma is easy to perform with less bleeding intraoperative, shorter opration time, less pain postoperative, coblation by low-temperature plasma in tonsillectomy expectively better in teenages group. It is worthy of popularization and application in children.

To understand the regulation of adenovirus pneumonia children procalcitonin through the contrast of adenovirus pneumonia and non-adenovirus respiratory tract viral pneumonia in children, common adenovirus pneumonia in children and severe adenovirus pneumonia in children with serum procalcitonin levels.

Chikdren patient with the diagnosis of pneumonia in the department of pediatrics of our hospital during Jan 2015 to Jul 2016 were enrolled. The patients were divided into groups with detection of adenovirus pneumonia and group with non-adenovirus infection of respiratory tract virus pneumonia. Serum calcitonin concentration was taken for two sample t test, and calcitonin increasing rates of the two groups was taken for chi-square test. The adenovirus pneumonia group was divided into common adenovirus pneumonia group and severe adenovirus pneumonia group, the calcitonin concentration of the two groups was taken for the two sample test.

The serum calcitonin concentration and calcitonin increasing rate in adenovirus pneumonia group were significant different with that in group of non-adenovirus infection of respiratory tract virus pneumonia. Adenovirus pneumonia in calcitonin concentration (t=19.696, P=0.001) and calcitonin increasing rate (χ²=19.696, P=0.002) were signifcantly higher than those in non-adenovirus infection of respiratory tract virus group. There was significant difference in calcitonin concentration between common adenovirus pneumonia group and severe adenovirus pneumonia group (t=12.696, P=0.001). Severe adenovirus pneumonia group was significantly higher than that of common adenovirus pneumonia group.

The procalcitonin concentration of the adenovirus pneumonia in children is significantly higher than that of the non-adenovirus respiratory tract v

Methylenetetrahydrofolate reductase C677T and A1298 Cpolymorphismsreduce the ability of synthesis methyltetrahydrofolate. So the methyltetrahydrofolate supplement pregnancy outcome was compared tothe folic acid supplement in methylenete trahydrofolate reductase polymorphisms.

The 80 participants were women with recurrent abortion, aged 35 to 40 years, methy lenetetrahy drofolate reductase C677T and A1298 Cpolymorphisms. The cases were randomly assigned to receive either folic acid or methyltetrahydrofolateaccording to the stratified blocked randomization by age, the number of previous abortions and methylenetetrahydrofolate reductasepolymorphisms. Participants took daily 800 μg methylentetrahydrofolate or 800 μg folic acid from at least 12 weeks before conception to the 24 th week of the pregnancy. Pregnancy complications and preganacy outcome of two groups were followed-up.

After an average of 40 weeks of tracking, 32 cases of pregnant women in the folic acid group delivered their babies full term, 4 cases premature delivery, 2 cases early miscarria ge and 1 case late abortion. In the methylenetetrahydrofolate group, a case was dropped, 30 cases of pregnant women delivered their babies full term, 5 cases premature delivery, 2 cases early miscarriage, 1 case late abortion. There was no significant difference in preganacy outcome between two groups.

The results don't support any beneficial effect of 5-MTHF and folate supplementation inwomen with recurrent abortion with any MTHFR C677T and/or A1298C polymorphism.

To investigate the protection of Annexin A1 on the injury of intestinal protective effect of propofol in rats with sepsis.

Sepsis of rat model was induced by injection of lipopolysaccharide in rat femoral vein. The rats were randomized to 5 groups: normal control group(Group C), LPS group(Group L), propofol group(Group P), LPS+Propofol group (Group L + P), Intralipid group(Group I), LPS + Intralipid group(Group L + I). The intestinal tissue in each group were stained with hematoxylin and eosin(HE) and examined under microscope. The expression of tumor necrosis factor(TNF-α), interleukin1(IL^-1) and leukocyte mediated endothelin-1(IL-6) on intestinal tissue were detected by ELISA. Western blot was used to detect the expression of Annexin A1.

Compared with the control group, the expression of IL^-1, IL-6, TNF-α was significantly increased, the Annexin A1 was obviously decreased in group L, while the opposite in group L+P.

Propofol may have protective and anti-inflammatory effects on the intestinal tract of rats with sepsis, and the effect may be correlated with the Annexin A1.

To explore the relationship between expression level of aquaporin-4 (AQP4), protein kinase C (PKC) and brain edema based on the focal cerebral ischemia reperfusion rat model.

The rat model of middle cerebral artery (MCA) was duplicated by suture method .The rats were randomly divided into normal control group, sham-operated group and cerebral ischemia reperfusion(CIR) group. Water permeability for rat astrocytes was evaluated by the green fluorescent protein(GFP) labeled AQP4 tracing system. Scores of neurofunctional defect were graded and water content in brain tissues was measured. Expression of AQP4 and PKC in brain tissue was detected by immunohistochemistry.

Neurofunctional defect occurred at 6 h after CIR. Water content in brain tissue increased at 12 h after CIR, reached peak at day 1-3. The expression levels of AQP4 were decreased first, then increased gradually at 12 h later, obviously increased at 24 h after CIR and reached to highest point at day 3. While PKC increased gradually at 6 h after CIR, kept high level for 5 days.

Brain edema may relate to the rise of PKC and AQP4, PKC may reduce the expression of AQP4 via phosphorylation in the early stage of CIR in order to delay the process of brain edema.

To observe the effects and differences between the two amplification instruments combined capillary electrophoresis.

DNA extracted were amplified with the optimized PCR method and cycling protocol using Powerplex 21 kit on the Applied Biosystems 9700 and HEMA 9700 instrument, including direct amplification and DNA amplification, and then genotyped using Applied Biosystems 3130xl. Same DNA test was repeated five times to test the stability of the instrument, seven kinds of non-human DNA samples were detected and 9947 was used as positive standard to test the sensitivity. Then genotyping results were validated and discussed.

Genotyping results between the two instruments had no significant differences, genotyping results were stable and accurate and showed consistency in species-specific detection and sensitivity detection between the two amplification instruments.

There is no significant difference between the two amplification instruments of the routine amplification test and the results were consistent. The results showed that the two amplification instruments could be used in routine amplification.

To explore the effect of unprotected perineum delivery technique in spontaneous vaginal delivery in low risk primipara.

From January to March 2015 ,182 primipare with spontaneous vaginal delivery in the hospital with no episiotomy indication were randomly divided into observation group (n=86) and control group (n=96).Unprotected perineum delivery technique and routine delivery technique were adopted in the observation group and control group after the fetal head crowned. Perineal integrity rate, the degree of perineal laceration,perineal edema,the hemorrhage volume 2 h after delivery, newborn asphyxia situation were compared in two groups.

Theperineal integrity rate was higher and perineal edema rate was lower in observation group than that in control group（P＜0.05）. No significant difference was detected in the degree of perineal laceration, the hemorrhage volume 2 h after delivery, newborn asphyxia situation between the two groups（P＞0.05）.

Unprotected perineum delivery technique can decrease the degree injury of perineum, with no labor complications.

To explore a new aptamer-based rapid detection method for lung cancer tumor markers.

Highly specific adaptive were screened by using systematic evolution of ligands (SELEX) and affinity were detected by flow cytometry. So as to choose a high affinity and high specificity of aptamer. Gold nanoparticles were prepared through sodium citrate reduction. The method based on label-free analysis of gold nanoparticles and aptamers were established, with methodological investigation.

High specificity and strong affinity of 3 NSE were obtained through 6 rounds of screening, with highest specificity of a. A rapid method for the detection of NSE antigen were established through the selection of tumor markers of lung cancer and gold nanoparticles.

The method has a good accuracy for the early detection of lung cancer provides a new means.