To discuss the effects of breathe anesthesia on experimental and clinical significance in 7.0 T animals magnetic resonance (NMR) studies.

Selected 80 SD rats, each 130 g, used oxygen (2.0 lps) plus isoflurane (0.5%) for the induction of anesthesia. After anesthesia completely, moved the rats to check bed, then devided the rats into 4 groups according to the continuous anesthesia dose (0.1%, 0.15%, 0.2%, 0.25%), with 20 rats in each group, for T2 WI imaging in rat's abdomen.

In the experiments, 4 rats were death, 15 used a second anesthesia and 76 rats completed the experiments, compared with A、B、D groups, group C was best in the security and practicability, the difference was statistically significant.

In the experiments, the quality of breathing can directly affect the quality of the images, the experiment process and results have a direct impact to the diagnosis of lesions. Proper anesthesia dose can effectively control the status of the experimental animals, improve the quality of the experiment and provide experiment protection for clinical diagnosis.

To explore the correlation between Th17/Treg balance and the grades of hydatidiform mole.

60 patients with collecting hydatidiform mole (light weight each in 20 cases), 20 cases of velvet carcinoma were collected at the same time as the positive control, and collect the same age of 20 healthy women as a negative control. Continue to qing dynasty palace and decidual tissue in patients with hydatidiform mole, and in the peripheral blood, using flow cytometry test peripheral blood and decidua in Th17 cells and intracellular cytokines IL-17, IL-23, and its function factor ROR gamma t content; Also using flow cytometry to detect Treg and intracellular function factor IL-10, TGF-beta 1 and percentage of Foxp3.

The patients with hydatidiform mole only IL-and there was no significant difference with normal, Th17, IL-17 and ROR gamma t and velvet carcinoma patients were significantly lower than normal (P < 0.05). Velvet carcinoma Treg, IL-10, TGF-beta and Foxp3 were significantly higher than that of hydatidiform mole patients and normal people (P < 0.05). Between the three groups of Th17/Treg balance to hydatidiform mole patient minimum, significantly lower than the other two groups, two differences between the three groups were statistically significant (P < 0.05). As the lesion severity is aggravating, hydatidiform mole patient peripheral blood and decidua in Treg number increasing, and the diminishing of Th17/Treg and Th17; Peripheral blood and decidua Treg classification is significant positive correlation with pathological changes; And of Th17/Treg and Th17 and classification show significant negative correlation, the related degree of Th17/Treg maximum balance relations with severe (above the absolute value of the r value is greater than 0.7, P value is less than 0.05).

The decrease Th17 cells in the peripheral blood and decidua, Treg increased, resulting in smaller ratio of Th17/Treg to Treg tilt, hydatidiform mole is an important phenomenon of immunology, and with the severity of the hydatidiform mole has significant correlation.

Discussion on early postpartum pelvic floor musclescreening and three-dimensional ultrasound role and clinical significance.

Selected 2012 September to 2014 June in Qingyuan People's Hospital hospital delivery, 600 cases of primipara postpartum 6 weeks, one group of 350 cases of vaginal delivery, cesarean section group 250 cases take over the same period, gynecological clinichealthy nulliparous women 100 cases as control group, separately carried on the pelvic floor muscle strengthscreening and three-dimensional ultrasound transperinealdetection three group, anal contraction movements in resting and pelvic floor hiatus and levator ani muscle thickness.

According to the standard of the pelvic floor muscle poweris divided into 6 levels, the IV level and above for pelvic floor muscle strength is normal, a total of 92 cases (15.33%), 0-grade III is not normal, a total of 508 cases (84.67%); wherein the cesarean section group normal rate was 33.6%, the rate of normal vaginal delivery group was 2.29%, control group of normal rate 100%; the difference was statistically significant vaginal delivery and cesarean section group; the first two group and control group had significant difference. Vaginal delivery and cesarean section group in resting and anal contraction movementsunder the condition of pelvic diaphragm hiatusanteroposterior diameter, transverse diameter and area were higher than that of the control group (P < 0.05), levator ani muscle thickness were less than that in control group (P < 0.05).

Pelvic floor muscle strength screening combined with 3D ultrasound measurements provide for the diagnosis ofpostpartum pelvic floor dysfunction of the anatomical and functional aspects of the basis and for formulating guiding individual pelvic floor rehabilitation treatment to provideoptimal treatment scheme.

To explore the Clinical Significance of Microwave therapy in the rehabilitation after appendectomy.

Select in patients after appendectomy 98 cases, divided into two groups randomly; 52 patients in the treatment group, control group of 46 cases, Treatment group with abdomen microwave hyperthermia after surgery in 24h; A comparison was done between this two groups after surgery with recovery time of bowel sounds, anus exhaust, abdominal distension, the incision pain and analgesic use, the incision fat liquefaction and infection, incision healing duration and hospital stay.

The recovery time of bowel sounds, anus exhaust, incision healing duration and hospital stay in treatment group were significantly shorter than those in control group (P < 0.001), The abdominal distension, the incision pain, analgesic use, the incision fat liquefaction and incision infection were lower than those in control group (P < 0.05).

Microwave therapy can reduce postoperative complications after appendectomy and benefit patients'postoperative recovery, which is a safe and feasible therapy, it worthy to be applied to clinic.

To introduces a simple preparation method of paraffin-embedded cell block and cell microarray and verify its application value.

Collecting clinical cytology specimens and then making paraffin embedding cell block and cell microarray by manual and verify its application value by HE staining, immunocytochemistry staining and TUNEL technique.

This method can produced cell block and about 60 lattice cell microarray. The dyeing effect of cell block and cell microarray are reliable confirmed by HE staining, immunocytochemistry staining and TUNEL technique.

As the manual preparation method of paraffin-embedded cell block and microarray required are simple and easy to operate, the method can be maintained well cellular morphology and antigenicity and worth popularizing and applying.

To improve the diagnosis and treatment of subacute thyroiditis with mass or nodule in surgery.

Thirty-eight cases of subacute thyroiditis were reviewed in the clinical and pathological data and restudied their pathological sections. Among them, two cases who accompanied with papillary thyroid microcarcinoma were processed by immunohistochemical marks.

The patients included 3 cases of males and 35 cases of females in a course of disease from 2 days to 2 years and 8 months. Among them, 28 cases were in 30-60 years old (73.68%). Every patient complained about masses or nodules in thyroid glands. There were no uncomfortable symptoms in 28 cases with subacute thyroiditis. The other 10 cases manifested pain or tenderness in the anterior region of the neck, sore throat, hyperthyroidism, fever and choking and so on. The diagnoses before operation contained subacute thyroiditis in a case (2.63%), suspected thyroid cancer in 2 cases, thyroid tumor in 6 cases, nodular goiter in 11 cases, thyroid mass in 18 cases. The diagnoses of frozen section during operation had subacute thyroiditis in 38 cases (accuracy in 100%). Among them, 10 cases of subacute thyroiditis with nodular goiter accompanying with 2 cases of papillary thyroid microcarcinoma. The pathological diagnoses confirmed subacute thyroiditis in 28 cases (73.68%); subacute thyroiditis with nodular goiter in 8 cases (21.05%); papillary thyroid microcarcinoma with subacute thyroiditis and nodular goiter in 2 cases (about 5.26%). Treatment: All cases had been treated by thyroidectomy. Among them, 10 cases were administered prompt medicine with good prognosis. There were 33 cases who were followed up from 1 year and 2 months to 9 years and 6 months after operation. Twenty eight cases with unilateral or bilateral subacute thyroiditis recovered very well in normal life and work. Five cases with bilateral subacute thyroiditis and nodular goiters had hypothyroidism and needed medicine in long term.

The patients with subacute thyroiditis in its remission and recovery period often go to the department of surgery because of thyroid mass or nodule and are apt to misdiagnosis. Frozen section during operation is important to confirming the resection extent, avoiding excessive resection and getting good treatment effectiveness.

To study the imaging features of Giant cell tumor of tendon sheath (GCTTS), in order to improve the recognition and the accuracy of imaging in the diagnosis of the disease.

14 cases with surgery and pathology proved GCTTS were retrospectively reviewed. 6 patients were carried out X-ray plain film, and 13 had plain and contrast-enhanced MR imaging.

X-ray plain film findings were soft-tissue swelling without osseous involvement or slight bone erosions. At MR imaging, the lesions were low signal and can be seen in the strip lower signal intensity on T1 weighted images, and the mixed signal intensity on T2 weighted images. The signal intensities tended to be moderately and heterogeneously enhanced following Gd-DTPA administration. The lesions were typically located in relation to a tendon or joint closely, local bone cortex involvement.

The imaging findings of GCTTS have some certain characteristics.

To investigate the clinical of schizophrenia and affective (mood disorders) causes of misdiagnosis between mental disorders.

in our hospital from April 20, 2010 to September 10, 2012 of schizophrenia and affective disorders in 33 cases, by the deputy director 2 psychiatrists and 1 senior attending psychiatrist Chinese to classification and diagnostic criteria of mental disorders (CCMD-3) to confirm.

Schizophrenia misdiagnosed as affective disorders in 12 cases (36.36%), misdiagnosis of affective disorders as schizophrenia in 21 cases (63.64%).

the misdiagnosis of mental illness and psychiatric symptoms do not know much about history, resources investigation, could not be less detailed course of evolution process analysis and three rounds does not improve the.

To analyze the availability and security of hysteroscopic and B-ultrasound treatment for difficult extracting IUD cases. Discuss the significant role of the B-ultrasound.

124 cases, which were difficult in taking out IUD with routine method, were operated by monitoring with B-ultrasound guides.

121 cases were removed by hysteroscopy and B-ultrasound successfully.

Combining use of hysteroscopy and B-ultrasonography is an effective and safety method to manage the difficulty extraction of IUDs.

To evaluate the clinical diagnosis of diffuse connective tissue disease patients by antinuclear antibody test Results.

Methods a retrospective analysis of 246 cases of diffuse connective tissue diseases and 160 cases of non diffuse connective tissue disease detection in hospitalized patients with autoantibody.

In a variety of diffuse connective tissue disease antinuclear antibody positive rate between the difference has statistical significance, in systemic lupus erythematosus patients, the positive rate of anti dsDNA antibody was 44.1%, the positive rate of anti Sm antibody positive rate was 47%; in mixed connective tissue disease in anti UIRNP antibody was 96.4% in patients with Sjogren syndrome; syndrome of anti SSA and anti SSB antibody positive rate was 68.2% and 59.1%; in patients with systemic sclerosis in anti Scl-70 antibody positive rate was 41.4%; overlap syndrome, anti dsDNA, anti Sm, anti UIRNP, anti SSA antibody positive rate was 45.4%, 18.2%, 45.4%, 18.2%.

There are important in the differential diagnosis of antinuclear antibody spectrum detection of diffuse connective tissue disease.

To investigate the effects and mechanism of breast cancer metastasis suppressor 1(BRMS1) in IL-1β secretion of fibroblast-like synovial cells (FLS) in rheumatoid arthritis (RA).

The synovial membrane tissues were obtained from 4 RA patients and 1 healthy subject and FLS were isolated by means of tissue culture. FLS were transfected with lenti-virus with BRMS1 protein. BRMS1 protein and nuclear protein NF-κB were detected by Western blot, IL-1β secretion was estimated by Elisa and nuclear translocation of NF-kB was observed by immunofluorescence.

The BRMS1 protein was obviously decreased in RA group than normal group, but nuclear protein NF-κB and IL-1β secretion were increased significantly. Nuclear protein NF-κB and IL-1β secretion were obviously downregulated in the FLS with BRMS1 protein overexpression of RA group. LPS induced nuclear translocation of NF-κB and IL-1β secretion were obviously inhibited by BRMS1.

BRMS1 could suppress the nuclear translocation of NF-κB and then reduce the IL-1β secretion of FLS.

We studied the effect of intensive atorvastatin treatment on carotid arterial intima media thickness (IMT) and plaque area (PA) in coronary artery disease (CAD) patients receiving percutaneous coronary intervention (PCI) in the present study.

120 CAD patients were randomly divided into two groups in our study. The carotid arterial IMT and PA before and 1 month, 6 months after PCI were then measured.

The carotid arterial IMT and PA significantly decreased in the intensive atorvastatin treatment group compared to conventional treatment group [One month after PCI, IMT, OR=0.62, 95% CI (0.13-3.05), P=0.015; PA, OR=0.78, 95% CI (0.09-1.69), P=0.041. Six months after PCI, IMT, OR=0.58, 95% CI (0.10-2.90), P=0.013;PA, OR=0.70, 95% CI (0.11-1.78), P=0.035].

The carotid arterial IMT and PA would be significantly reduced for the CAD patients receiving PCI who has been given the enhanced atorvastatin treatment.

To explore leg fracture minimally invasive operation treatment, in order to use minimally invasive treatment of fracture.

86 cases with fracture of nearly 2 years of the calf, which use minimally invasive treatment of leg fracture 46 cases analysis, early functional exercise for postoperative, regularly X ray, monitoring of fracture healing, according to the situation and put forward guiding function exercise.

BO theory of fracture healing, micro invasive treatment of leg fracture is a kind of effective fixation method, compared with the traditional AO open operation, reduce the injury of soft tissue, to reduce fracture of local soft tissue and periosteal blood supply destruction, provides an ideal tissue repair of environmental biology, shorten operation time, reduce bone nonunion and infection, is conducive to the functional recovery of patients after operation, satisfactory clinical effect.

To explore the influence of systematic nursing intervention on illusion-delusional state hospital nursing effect of patients.

from 2013~2014 October in our hospital treatment of illusion -patients with delusional state in 60 cases, were randomly divided into observation group and control group, 30 cases in each group, the control group with routine psychiatric nursing, the observation group with systematic nursing intervention, stay in the hospital for observation for 6 months, with the positive and negative symptom scale and self evaluation of clinical efficacy scale of two groups for statistical analysis and comparison.

the two groups in total score of PANSS, PANSS positive and so on have markedly improved, there is obvious difference compared with before entering the group (P < 0.05); observation group in PANSS total score, PANSS positive and so on improved significantly better than the control group, the difference was statistically significant; the observation group had higher efficiency than the control group, the P < 0.05, with significant difference.

systemic nursing intervention can significantly improve the hospitalized patients withhallucinations -delusional state, play a positive role in clinical.