To investigate cell-free DNA on trace touch DNA samples and the amount and proportion of the cell free DNA and the intracellular DNA and the influence of cell-free DNA in forensic analysis.

To manufacture mock exhibits and then the DNA extracted from the samples was quantified using standard manufacture's procedures, and the results were analyzed and discussed.

This experiment shows that the cell free DNA has been detected in 78.55% of the samples. The detection rates in hand touch DNA samples, face touch DNA samples, foot touch DNA samples and mouth-swab respectively are 82.1%,82.1%,53.6% and 92.4%. The amount of cell free DNA is 12.9% of the amount of total DNA in trace/touch DNA samples.

The cell free DNA did exist extracellular, and the amount of the cell free DNA occupy a proportion in the exhibits of touch DNA. This study showed a new theory of the question of cell free DNAof touch DNA.

To observe the effects of Propofol on membrane characteristics and postsynaptic current of hippocampal pyramidal neurons.

The method of frozen section cut hippocampus into 300um thickness in C57 mice. Using whole cell patch clamp technique records the hippocampal neuronal responses of action potential(AP), I-V curves and postsynaptic current during using propofol before and after.

Propofol decreased significantly the number of action potential under different stimulus intensity before (5±3) and during (2±1) propofol application(P<0.01), but had no significant effect on the AP amplitude. Zeneca may change the hippocampal pyramidal neurons plateau reaction to excitatory voltage stimulus in I-V curve, the max current amplitude increased from（1647.63±124.02）pA to（2955.08±119.10）pA(P<0.01). Propofol can decrease the pyramidal neuronal postsynaptic current from（146.5±25.89）pA to（72.8±18.71）pA(P<0.01), and then return to（132.1± 30.2）pA (P<0.01)after washing 20 min.

Propofol can reduce hippocampal pyramidal neuronal AP number, change the I-V curve plateau responses and decreased reversibly postsynaptic currents.

To evaluate the effect of inhibition Heat shock protein 90 (HSP90) on apoptosis in esophageal squamous carcinoma cell TE-1.

TE-1 were treated with different time（24 h, 48h or 72 h）and different concentrations（0.25 μmol/L, 0.5 μmol/L, 1.0 μmol/L or 2.0 μmol/L）of 17-allylamio-17-desmethoxygeldanamycin (17-AAG). The cell proliferation, apoptosis and protein expression of Hsp90, Hsp70, Akt and Fas were determined by MTT method, flow cytometry and western blot.

The 17-AAG on TE-1 cell in vitro proliferation inhibition and apoptosis rate had obvious promoting effect, and time-dose dependent. After curcumin treatment, the protein expression of Hsp90 and Akt significantly reduced, Hsp70 and Fas significantly increased.

17-AAG can significantly inhibit the proliferation of TE-1, promote apoptosis, its mechanism may be through inhibiting Hsp90 activity and influence the related signaling pathway.

To investigate the role of the foldable capsular vitreous body (FCVB) in mechanically releasing nerve growth factor (NGF). Our study supplies the experimental evidences for further investigation contributing in intraocular drug deliver systerm exploration.

We choose in vitro study. NGF sustained-release studies in vitro was finished in cups of modi fied Franz diffusion cells. Concentration of 3 ug/ml NGF diluted in steril water was injected into the FCVB capsules, immersed into small cup with 15 ml steril water. Two hundred (200) μl liquid was aspirated at time intervals of 30 min, 2, 4, 8, 12 h separatively. Standard curve was made through ultraviolet spectrophotometry examination. The assumption length of NGF examination was 280 nm.

The data from in vitro study indicated that FCVB could mechanically release NGF in dose and time dependent manner. At 12h time point, NGF was released at concentration of 465ng/ml.

s Data from in vitro study showed that FCVB could sustainably, mechanically release NGF via capsule apertures in a time-dependent and dose-dependent manner. This research for the further study can confirm FCVB built intraocular drug sustained-release system to provide the basis for clinical at the same time corresponding treatment of diseases.

To explore the relationship between the change of ratio caudate lobe/right lobe of the liver and diameters in subjects with healthy livers and subjects with nonalcoholic fatty liver disease（NAFLD）.

the following ultrasonic measurements of caudate lobe and right lobe of the liver were performed in 374 adult subjects: 227 with healthy livers and 147 cases with NAFLD diagnosed in the Lecong Hospital: a: the upper and lower diameter, b: the anteroposterior diameter, c: the transverse diameter of caudate lobe of the liver and d: the transverse diameter, e: the maximum oblique diameter of right lobe of the liver were measured and compared the following ratios were calculated: c/d and a/e.

Across all measurements there were significant differences (P<0.05) thus the sizes ranked from severely>moderately>mildly fatty liver>healthy liver. The ratio between c) (the transverse diameter of caudate lobe of the liver) and d) (the transverse diameter of right lobe of the liver and Ratio a) the upper and lower diameter of caudate lobe of the liver and e) (the maximum oblique diameter of the right lobe the liver) in different grades NAFLD were not significant differences.（P>0.05）.

the change of diameters of the caudate lobe and the right lobe of liver is closely correlated with different degrees of NAFLD,The changes in ratios of the caudate lobe and the right lobe of liver are not very closely correlated with different degrees of NAFLD,consequently the ratios are of limited value as diagnostic indicators.

To explore and analyze the advantage of off-pump coronary artery bypass (OPCAB) compared with conventional coronary artery bypass (CCAB).

88 cases of CCAB and 145 cases of OPCAB were collected from Nov. 1999 to Dec. 2006, and the clinical data were summarized and analyzed retrospectively.

The preoperation risk factors were no significance between CCAB group and OPCAB group except for diabetes. There were more mean graft numbers in CCAB group than in OPCAB group. But operation time, intubation time, ICU stay time, postoperative stay time, low cardiac output, IABP use time and renal dysfunction in OPCAB group were obviously less than in CCAB group, no significance in hospital death between the two groups. Three years postoperative follow-up result indicated that heart function improved obviously and EF value was higher in OPCAB group than in CCAB group.

There was apparently advantage in postoperation recovery and reduction of postoperation complications in OPCAB compared with CCAB, and OPCAB is a hopeful surgical method for coronary artery disease.

To investigate the relationship between angiotensin converting enzyme (ACE) gene I/D polymorphism and atherothrombotic infarction.

112 patients with atherothrombotic infarction and 180 age, sex-matched healthy subjects were recruited. Genotypes were detected by PCR-AFLP.

Univariate analysis revealed that systolic blood pressure (SBP) levels for patients (138.1 ± 19.7mm Hg) were significantly higher than those for healthy controls (126.6 ± 20.6 mmHg) (t=4.716, P<0.001), and diastolic blood pressure (DBP) levels for patients (88.9±10.1 mmHg) were significantly higher than those for healthy controls (79.9 ± 10.0 mmHg) (t=7.450, P<0.001). Allele D frequency and distribution of frequency for genotype DD of ACE gene I/D polymorphism for patients were significantly higher than those for healthy controls (allele: x2= 4.953, P=0.026; genotype: x2=6.303, P=0.043). In both groups, the SBP and DBP levels for genotype DD were significantly higher than those for genotype ID and II.

Allele D of ACE gene I/D polymorphism plays a role in elevating blood pressure and may be a major risk factor for atherothrombotic infarction.

To study the clinical efficacy of combining tibiofibular anterolateral approach with external fixation on the Gustilo III distal tibiofibular fractures.

70 patients with Gustilo III distal tibiofibular fractures were selected and divided into the research group and the control group according to the operative plans. The research group were treated with tibiofibular anterolateral approach combined with external fixation while the control group were treated with open reduction combined with internal fixation. The general operation conditions (time of operation, intraoperative quantity of bleeding, incision complication, etc) of two groups were observed. According to the scores of AOFAS, the therapeutic effect were evaluated before and after surgery.

The operation time and intraoperative quantity of bleeding of the research group were respectively（72.3 ± 9.6）min and（81.3 ± 9.5）ml while the operation time and intraoperative quantity of bleeding of the control group were respectively（86.3±10.3）min and（103.3±11.9）ml (P<0.05);The incidence rate of incision complication of the research group (2.86%) was significantly lower than that of the control group (17.14%) (P#60;0.05). According to AOFAS, the scores of ache, function and alignment as well as total points of the research group were respectively（34.5±7.5）,（42.7±3.2）,（9.5± 1.9）and（86.3±9.6）while the scores of ache, function and alignment as well as total points of the control group were respectively （31.6 ± 5.7）,（32.3 ± 7.0）,（8.2 ± 2.2）and（72.5 ± 13.7）.（P<0.05）.

The clinical efficacy of applying the tibiofibular anterolateral approach with external fixation to treat Gustilo III distal tibiofibular fractures is better than that of the treatment with open reduction combined with internal fixation, which could relieve the trauma for the patients and reduce the incidence rate of complication. It is worthy of promotion.

We aimed to analyze the relationship between isolated short femur in the second trimester and pregnancy outcomes.

We collected 247 patients with a second trimester ultrasound (16-27weeks) from July 2013 to July 2014. Based on the femur length measurement, patients were divided into the normal femur length group and the short femur group. Clinical data and pregnancy outcomes of patients were compared.

232（93.9%）had normal femur length, 15 （6.1%）had short femur. Mothers in the short femur group were shorter and had a lower pregnancy and delivery weight than the group with normal femur length（P<0..05）. Tobacco use during pregnancy was more frequent in the short femur group (P<0..05). Small for gestational age, low birth weight and preterm birth occurred more frequently in the short femur group (P<0..05).

Fetal isolated short femur in the second trimester increases the risk of preterm birth, low birth weight and small for gestational age.

To study the dynamic change and clinical significance of CD4+CD25+ 的CD4+CD25+regulatory T cell for uremia patients undergoing hemodialysis.

To collect original uremia and maintain all the 80 cases of hemodialysis patients, for more than three consecutive patients with dialysis, using flow cytometry test peripheral blood Treg and intracellular transforming growth factor beta 1 (TGF-beta 1) and interleukin 10 (IL-10) percentage; And compare the renal function changes before and after dialysis; Will Treg, TGF-beta 1 and IL-10 and renal function and clinical indicators for correlation analysis.

The Treg healthy controls, TGF - beta 1 and IL-10 percentage were 4.27 +/-1.29, 2.89 and 3.76 1.13 mm, 0.93 mm was significantly higher than group and maintaining dialysis group (P<0.05). Was in dialysis three times, the Treg, TGF-beta 1 and IL-10 were significantly lower than maintaining group (P<0.05); With the progress of the dialysis, two groups of patients of Treg, TGF - beta 1 and IL - 10 were significantly decreased (P<0.05), and a decline in both the group is to maintain large group (P<0.05). BUN and Scr primary-care group were significantly higher than maintaining group (P<0.05). With the progress of the dialysis, the BUN and Scr in two groups of patients were significantly decreased (P<0.05), and a decline in both the group is to maintain large group (P<0.05). Time needed for both the uremia patients renal function back to normal and hospital stay were 14.16+/-5.16 and 11.39+/-6.84 days, significantly shorter than maintaining dialysis group 18.29 +/-7.72 and 15.69+ /-8.39 days (P<0.05). Correlation analysis suggests Treg and TGF-beta 1 and IL-10 and BUN, Scr and renal function back to normal the required number of days and hospitalization days were significantly negatively correlated (P<0.05).

The patients with uremia Treg, TGF-beta 1 and IL-10 declined significantly, with the progress of the dialysis three progressive decline, is closely related to renal function and clinical indicators.

To evaluate the curative effect of sorafenib tosylate in patients with hepatocellular carcinoma (HCC) via regulatory T cells.

The HCC patients were divided into control group and the beauty of messenger 30 cases, before and during treatment, treatment for 6 months and 12 months respectively detection HCC patients peripheral regulatory T cells (Treg) and cytokine interleukin 10 (IL-10) and transformation growth factor b1 (TGF-b1) content detection; Testing the alpha-fetoprotein (AFP) and alanine aminotransferase (ALT) content; 12 months follow-up calculation on survival situation.

1) treatment for 6 months later, two groups of the above three level is decreased obviously (P<0.05), and the treatment after December drop more significant (P<0.05). Comparison between groups, treatment after 12 months, beauty messenger Treg group, IL-10 and TGF-b1 were significantly lower than that in control group (P<0.05), and the messenger beauty group in the treatment of 6 months already was significantly lower than the control group.1) during the period of follow-up messenger beauty group of AFP and ALT levels were significantly lower than that in control group (respectively, P=0.000, 0.001). 3) the correlation analysis found that Treg, IL-10 and TGF-b1 between three parturients; More than any of these three things, and AFP and ALT are of parturients. 4) follow-up of 12 months to messenger beauty group 2 group of death, and control group for five people, but two groups of death number constitute no statistically significant (P=0.067), but the beauty of the messenger group 12 month average survival time was significantly longer than that in control group (P=0.041).

Toluene sulfonic acid sorafenib can inhibit the Treg and cell factor, and reduce the AFP concentration, this may be the drug with good clinical effect of the mechanism.

To conclude the imaging characteristics of the patients with pulmonary inflammatory pseudotumor who suffered from complications after puncture via CT scan.

analysis of 15 cases of complications puncture (group A) and 32 cases (group B) does not appear complications in patients with the clinical features and imaging characteristics of comparison to compare differences between the two groups.

in group A is given priority to with women, and group B is given priority to with men, two groups of gender constitute A statistically significant (P=0.036); Is given priority to with the youth group A and group B is given priority to with middle-aged, age differences between the two groups was statistically significant (P=0.002). Course significantly shorter than group B, group A false tumors had the largest diameter was significantly more than group B (P=0.033), and computed tomography (CT) value was significantly lower than that of group B (P=0.009). Give priority to with the left pulmonary pseudotumor, group A and group B with A bit more right lung, good hair lung side similar between the two groups was statistically difference (P=0.039); Most complete capsular, group A and group B most envelope (P=0.021). A group of patients with mediastinal lymph node enlargement is A bit more, and most of group B without lymph node enlargement (P=0.029). Most of the group A pseudotumor showed irregular shape, but group B most assumes the circular or nearly circular, shape difference was statistically significant (P=0.027); Pseudotumor most uneven density, group A and group B most homogeneously density, difference was statistically significant (P=0.032).

The onset time shorter young female patients with pulmonary inflammatory pseudotumor, especially in the left lung, tumors had larger diameter, irregular shape, uneven density, CT value is low, coated incomplete, associated with mediastinal lymph node enlargement, should be vigilant the complications caused by CT guided puncture.

Through the process of different imaging methods in the diagnosis of two cases of traumatic diaphragmatic hernia, get lessons learned, and to discuss and look for cheap, convenient and quick to, more accurately the method in the diagnosis of traumatic diaphragmatic hernia, at the same time avoid interference caused by patient can't cooperate, such as coma, be agitated, touch, etc.

Collect the images of two cases of traumatic diaphragmatic hernia and clinical data, operation: In one case, because the doctor on duty to consider is not sufficient，only the chest and abdomen CT scan + diaphragm thin layer 3 d reconstruction; Another case after taking full account of the chest and abdomen CT examination（CT scan + diaphragm thin layer 3 d reconstruction）+ X-ray fluoroscopy + X-ray radiography (leave medical image data). Then compare the diagnostic results of the two cases, the clinical observations and surgical results.

Clinical feedback and surgical results were confirmed, the diagnosis results of X-ray fluoroscopy diagnosis combined with clinical can be more quick and accurate diagnosis, and has its unique scientific nature.

Fully aware of the imaging findings of diaphragmatic hernia, skillfully master all kinds of differential diagnosis, seriously in combination with clinical symptoms and signs, for fast and accurate diagnosis of traumatic diaphragmatic hernia is not difficult, so as to more effectively reduce mortality, reduce complications and improve cure rate.

To discuss the value of echocardiography applied in apical hypertrophic cardiomyopathy(ApHCM).

Echocardiography and electrocar- diogram were performed in 23 patients with“pure”ApHCM．

In patients with ApHCM ,thickened apical segments produce a crowded small apical cavity with obliteration of the apex during systole. All patients had giant negative T-waves in the precordial ECG leads. During follow-up 4 cases had ApHCM-related cardiovascular events,None of the patients had sudden cardiac death.

Echocardio- graphy is the main method of diagnosing ApHCM and has important clinical value.

To investigate the effects of intracoronary and intravenous application of tirofiban on platelet aggregation rate(PAR) in patients with acute coronary syndrome(ACS).

Seventy-two patients with ACS undergoing percutaneous coronary intervention(PCI) were randomized into intracoronary tirofiban bolus group(group C, n=36)and intravenous tirofiban bolus group(group V, n=36). The changes of PAR at different time points after tirofiban application in two groups and the differences in the degree of PAR decrease between two groups were analysed, and the prevalences of bleeding and thrombocytopenia were also recorded.

PAR decreased after treatment with tirofiban in both groups，and there were significant differences between PAR after treatment and that before treatment(P<0.05). In coronary artery, AT 1 minute after the trentment the trend of PAR decrease in group IC was more significant than that in group V(P<0.05). In peripheral vessel, there was no significant difference in the degree of PAR decrease between two groups(P>0.05). There was no significant difference in the prevalences of bleeding and thrombocytopenia between two groups(P>0.05).

Both intracoronary and intravenous application of tirofiban can effectively decrease PAR in coronary artery and peripheral vessel, and may not increase the risk of bleeding．

To explore the value of PDI(Power Doppler Imaging）and elastograph in differential diagnosis between beast cancer and beast benign tumor by the detection of high frequency color Doppler ultrasound.

Selecting 92 beast tumors evidenced by the pathological results, observing blood characteristics of high frequency color Doppler ultrasonic PDI and elastograph , and compared with the postoperative pathological results, the differential diagnostic effects of blood characteristics and Elastograph have been estimated.

The sensitivity，specificity and accuracy of PDI in diagnosis of beast cancer were 66.7％. 71.4％. 69.6％. the sensitivity，specificity and accuracy of ultrasonic elastography in diagnosis of breast cancer were 80.6％, 85.7％, 83.7％,comparison of accuracy between the two methods, χ²=5.129，P<0.05, means ststistic significance can be found .

The accuracy of Elastograph is higer than PDI in differentially diagnosing beast cancer.

To evaluate the application of hysteroscopy in the diagnosis of early unexplained recurrent spontaneous abortion(EURSA）.

A retrospective study is performed to analyze the findings of hysteroscopic examination of 1359 EURSA cases who referred to our hospital during January 2010 to June 2014.

The occurrence of intrauterine abnormalities is 29.9%, of which, the intrauterine adhesion lie the first row(14.3%), and with the increasing times of abortion , the degree of intrauterine adhesion occur increase(P=0.003).Other intrauterine adnormalities are found including endometrial polyps(6.4%),endometritis（3.8%），uterus subseptus（2.7%），cervical incompetence（1.3%）, tuberculosis of endometrium（1.1%）, Pregnancy residues（0.4%）.The main complication is abortion syndrome(0.7%).

Hysteroscopy is a safe inspection for the patients of EURSA, which can reveal the unfavorable factors for pregnancy and is worthy of extension in clinical application.

To investigate the rheumatoid arthritis (RA) patients with secondary pulmonary interstitial disease (ILD) hormone impact CD8 + CD28 -T cells before and after the treatment with the change of meaning.

Included in the total of 23 patients with RA-ILD (group A), selection of RA patients during the same period of 25 cases (group B), the RA patients with ILD 25 cases (group C), 25 cases of healthy people (group D) as A control. For more than A group of patients with A prednisolone impact indications for treatment, using flow cytometry to detect the above four groups before and after the treatment of peripheral blood CD8 + CD28 - T cells and cytokines interleukin 10 (IL-10), and transforming growth factor beta 1 (TGF - beta 1) the percentage of change.

Compared with group D, A, B and C three groups of CD8 + CD28 - T cells, IL-10 and TGF - beta 1 were significantly increased, content from high to low were characterized by A>C>B>D, and four groups between any two sets of the above three indicators are statistically significant (P&#60;0.05). A group of patients after treatment of CD8 +CD28 - T cells, IL-10 and TGF-beta 1 than before treatment significantly reduced (P&#60;0.05).

CD8 + CD28 - T cells on the pathogenesis of rheumatoid arthritis secondary pulmonary interstitial lesions process may play an important role, and methyl prednisolone can significantly inhibit the cell activity, is expected to be as a connective tissue disease caused by pulmonary interstitial lesions was observed and curative effect evaluation index.

The contrast is not the same age paragraph the adrenal pheochromocytoma (APC) before and after surgery in patients with the change of blood pressure and urine almond acid.

48 patients with adrenal pheochromocytoma were retrospectively analyzed the clinical, the diameter of lesion, the course of the 24 h urine, blood pressure and vanilla almond acid changes.

Women in both groups, but the sex differences in the two groups have no statistical significance; Both groups are to the left, the more the distribution but also no statistical difference. Elderly group lesions is bigger than the diameter of young and middle-aged group (P=0.003), and the elderly group significantly longer duration of young and middle-aged group (P=0.026). Before the treatment, the young and middle-aged group of systolic pressure, diastolic blood pressure and urine almond acid were significantly lower than the elderly group. After surgery, two groups of systolic pressure, diastolic blood pressure and urine preoperative significantly reduced almond acid than themselves, and were in young and middle-aged group was significantly lower than elderly group.

Large diameter, long course of disease is an important characteristic of elderly APC; Surgery can significantly reduce the patient's blood pressure and urine APC almond acid content.

To explore the clinical value of serum lactic dehydrogenate(LDH), tissue polypeptide specific antigen (TPS), carcinoembryonic antigen(CEA),andβ2-microglobulin(β2-MG) in the diagnosis of non-Hodgkin,s lymphoma(NHL).

The serum level of LDH was determined with automatic biochemical analyzer and TPS,CEA and β2-MG levels were determined by enzyme-linked immumosorbent assay(ELISA) in 62 NHL patients ,8 lymphatic neagative patients and 40 healthy persons.

(1)The serum levels of LDH、TPS、CEA and β2-MG in NHL patients were higher than other groups （P<0.05）; (2)There was no statistical significance in examining the serum levels of LDH、TPS、CEA and β2-MG in NHL patients in high malignancy group, middle malignancy group and low malignancy group(P>0.05).

The detection of serum LDH、TPS、CEAand β2-MG is of value in diagnosis in patients with NHL.

To talk about femonston frozen thawed embryo transfer cycles in patients with poor endometrial development in application.

48 cases of endometrial dysplasia patients given femonston hormone replacement therapy regimens in the treatment of 38 cases of transplantation, complete cycle of patients according to whether the implantation success divided into implantation group and the non implantation group, changes of endometrial thickness were compared between the two groups after administration of general information and.

The general data such as endometrial thickness was no significant difference between two groups of patients with age, the cycle before. 9 days after the implantation of medication group and transplantation on endometrial thickness were greater than non implantation group, but no significant difference between two groups (P>0.05); endometrial implantation group to increase the thickness is greater than the non implantation group, with statistically significant difference (P<0.05).

Endometrial thickness increase may be an important factor in image implantation success rate, but with femonston in promoting endometrial dysplasia in patients with endometrial receptivity to change aspects of ideal effect.

To study the type and three atractylodes pill for pixu (spleen deficient) the effect of postoperative abdominal lymph node metastasis in patients with cervical cancer.

The syndrome differentiation for pixu (spleen deficient) and three atractylodes pill 26 examples of cervical cancer patients, 28 cases in control group patients did not use the drug, the follow-up 3 years, compared two groups of enhanced CT in patients with abdominal lymph node metastasis and survival rate.

Abdominal lymph node metastasis group is the first time interval significantly longer than control group (P=0.011), number of lymph nodes metastasis significantly less than the control group (P=0.009), and 3 year survival rate was significantly higher than that of control group (P=0.036).

Ginseng three atractylodes pill can slow type cervical cancer patients with postoperative abdominal lymph node metastasis of pixu (spleen deficient) time, and reduce the degree of invasion.

To study the application of three dimensional visualization technology for laparoscopic resection of cystic carcinoma in pancreatic body and tail.

Six cases of cystic carcinoma in pancreatic body and tail are retrospectively analyzed by reviewing clinical data from Nov, 2009 to Mar, 2011. All the patients’original image data of 64-slice spiral CT are completely collected. Using adaptive region growing algorithm, the serial CT images are segmented and automatically extracted, further we get the 3-dimensional reconstruction mages with the image manipulation software (developed by Fang Chihua). According to the reconstruction, we make the specific surgical approach (the trocar position), surgical procedure, etc.

The 3-dimensional reconstruction mages clearly presents the cystic carcinoma in pancreatic body and tail and the adjacent organs, especically for the relationship between the cystoma and the splenic artery and vein. All patients are successfully undergone laparoscopic resection of pancreatic body and tail, no perioperative death. Moreover, 5 patients preserve the spleen while 1 have to remove the spleen because of mucinous cystadenocarcinoma. The overall rate of pancreatic fistulae is 33.3%, no postoperative hemorrhage. The average hospitalization is 12 days.

Laparoscopic surgery has the advantage of minimal trauma, quick recovery and high percentage in spleen preserving, ect. The research of 3-dimensional reconstruction of pancreatic CT date is of great application value for laparoscopic resection of cystic carcinoma in pancreatic body and tail.

To explore the relation of essential hypertension and cognitive impairment in elderly people.

A total of 326 elderly patients with essential hypertention (with at least 5 years of history) from January 2011 to December 2013 were taken as observation group,and 300 healthy elderly people as the control group. The mini-mental state examination (MMSE) score were used to assessed cognitive function.

(1)The MMSE score of hypertention patients was significantly lower than that of control group (P<0.01). The prevalence of mild cognitive impairment(MCI)of hypertention patients was higher than that of control group (P<0.01). (2)MMSE score decreased with increasing blood level (P<0.01). Increasing prevalence of MCI was found in the subjects with high grade of hypertension (P<0.01). (3)The prevalence of MCI had a significant negative correlation with the grade of hypertension,LDL level and 2-hr plasma glucose.

(1)There is a close relation between hypertention and cognitive impairment in elderly pe ople.Seriousness of cognitive impairment is associated with high blood pressure. (2)The cognitive impairment in elderly people is associated with the grade of hypertention,LDL level and 2-hr plasma glucose.

This research was conducted to study the effect of Orem self-care theory and psychological nursing on tumor patients after chemotherapy.

140 patients from Radiotherapy Department of Nanfang hospital were randomly divided into treatment group and control group according to the sequence of admission. The control group was given regular grading nursing care, while the treatment group received Orem self-care and psychological nursing based on regular grading nursing care. We aimed to compare with the incidence of adverse reaction, the incidence of anxiety and depression and patients satisfaction with nursing service of two groups.

Compared with the control group，the treatment group had a lower incidence of gastrointestinal reaction (P<0.05), and had a lower incidence of anxiety and depression as well(P<0.05). In addition, the patients satisfaction of treatment group was 97.1% , higher than the control group which was only 73.3% .

Self-care and psychological nursing on tumor patients during the chemotherapy can significantly relieve the anxiety and depression and reduce the gastrointestinal reaction, while it can also improve the patients satisfaction with nursing service.

To establish a rapid method for detection of Group A Streptococcus (Strep-A), colloidal gold-based immune chromatographic assay (GICA) was used.

Based on the principle of double-antibody sandwich method, the colloidal gold strip has been developed. The specificity, sensitivity and clinical application were evaluated.

The lowest detection limit of assay was 105 CFU/mL and the detection time is about 10 min. The cross-reactions showed that there is no cross-reaction between test strip and group B Streptococcus, group D Streptococcus and staphylococcus aureus. In the 229 cases of clinical samples, the sensitivity and the specificity were 97.30% and 98.06% respectively. Kappa was 0.950 and consistency test showed no significant difference between results of traditional bacterial culture identification and test strip.

s This method was convenient, rapid and effective for the detection of pathogenic Group A Streptococcus (Strep-A). The development of GICA was applicable and laid a solid foundation for the rapid determination and screening of Strep-A in the field.