To achieve a novel macromolecules to traverse cell membranes by synthesis and characterization of a tumor- targeted ACPP and an ACPP modified- HPMA polymer conjugates.

Activable cell- penetrating peptide (ACPP) were prepared by free radical precipitation copolymerization method, the expression of mmp2 was measured by Immunofluorescence; the tumor-targeted cell-penetrating ability of ACPP was verified by inverted fluorescence microscopy; ACPP- pc- Ad.egfp conjugates was checked by inverted fluorescence microscopy and dynamic light scattering (DLS); Intracellular distribution of ACPP-pc-Ad. egfp was examined by inverted fluorescence microscopy.

Peptides ACPP was synthesized as devised and ACPP targeted and penetrated the tumor cells. inverted fluorescence microscopy and DLS assays demonstrated the formation of ACPP-pc- Ad.egfp conjugates; ACPP-pc- Ad.egfp showed strong ability to translocate into tumor cells exhibited nonendocytotic cytoplasmic delivery.

tumo- targeted ACPP was synthesized and modified the HPMApolymer successfully.

To investigate the effectiveness and safety of tandospirone combined with agomelatine treatment of Senile post- stroke depression.

61 cases of patients with Senile post- stroke depression were divided into treatment group of 31 cases, 30 cases in the control group , The treatment group was given tandospirone combined with agomelatine treatment, The control group was given agomelatine treatment; In treatment 2, 4 weeks, depression scale (HAMD), anxiety scale (HAMA) and side effects scale (TESS) assessment.

Clinical treatment of the two group of significant efficiency had significant difference (P＜0.05). Adverse reaction between two groups had no significant difference (P＞0. 05).

Tandospirone combined with agomelatine treatment of senile post-stroke depression curative effect, high safety.

To explore the outcomes of operation treatment for osteoporotic hip fracture in the elder.

117 patients with osteoporotic hip fracture were treated with operation methods between January 2000 and December 2010, which involved 53 males and 64 females, and with mean age 75.7±10.3 years(ranged from 65 to 95 year). 51 cases were suffered from interchanteric fracture, involved 24 males and 27 females, and with mean age 76.7±9.3 years(ranged from 66 to 95 years). All fractures in the cases were divided according to Evans standard, Ⅰtype in 4 cases, Ⅱtype in11 cases, Ⅲtype 32 in cases, Ⅳ type in 4 cases. 36 cases were treated by DHS, 5 cases by hollow pull screw, 6 cases by artificial bipolar femoral head replacement and 5 cases by Gamma screw. 66 cases were suffered from femur neck fracture, which involved 29 males and 37 females, and with mean 775.6±10.2 years(ranged from 65 to 92 year). All fractures were divided according to Garden standard, Ⅰtype in 5 cases, Ⅱtype in 13, Ⅲtype in 21 cases, Ⅳ type in 27 cases. 24 cases were treated by artificial bipolar femoral head replacement. 27 cases were by total hip replacement. 15 cases were by hollow pull screw.

44 cases with interchanteric fracture were followed-up for 8~26 months with an average of 15.7±3.3 months. The average fracture healing time was 4.6 months in the DHS cases. A few of complications happen in the DHS cases, screw cutting occurred in 3 cases. 2 cases suffered from infection dead due to cardio-pulmonary failure in 10 days after operation. 58 cases with femur neck fracture were followed-up for 8~25 months with an average of 16.7±2.3 months. According to Harris scoring system, the outcomes were excellent in 30 cases, good in 22, fair in 14, and poor in 1, and the excellent good rate was 91.38%(53/58).

Operation management can achieve an excellent result in osteoporotic hip fracture in the elder.

To observe the effect of metacortandracin administration method and time on bone metabolism in rats.

A total of 45 SPF rats were randomly divided into three groups (Normal group Intragastric administration group Injection group). Normal group, as a control group, administrated with nothing, Intragastric administration group with prednisone 10 mg/(kg · d). Injection group with prednisone 10 mg/(kg · 2d) , which were observed 12 weeks. All rat s were examined for bone mineral density (BMD), serum calcium，phosphorus, alkaline phosphatase.

after 12th weeks, compared with Normal group, BMD and serum calcium decreased significantly while the phosphorus, alkaline phosphatase increased obviously in Intragastric administration group and Injection group (P＜0.05). Compared with Intragastric administration group, BMD decreased significantly in Injection group (P＜0.05) while the level of serum calcium, phosphorus, alkaline phosphatase were not changed significantly (P＞0.05).

Compared with Normal group, both Intragastric administration and injection with prednisone could influence the level of BMD, serum calcium, phosphorus, alkaline phosphatase, while injection with prednisone could decrease the BMD more significantly, the research result shows some meaningful theroptic methods for the op patients.

To explore the clinical application effects of emergency exploratory laparotomy on the diagnosis and treatment of acute lower digestive tract hemorrhage associated with hemorrhagic shock.

The data of 36 patients with cute lower digestive tract hemorrhage from Oct 2005 to Jan 2012 in our hospital were reviewed. Among them, 15 cases (41.7%) combined with hemorrhagic shock were diagnosed and treated with sugery of emergency exploratory laparotomy.

13 patients (86.7%) combined with hemorrhagic shock were diagnosed definitely and cured timely except for 2 cases died from DIC.

Acute lower digestive tract hemorrhage associated with hemorrhagic shock should be intervented by operation as early as possible if the conservative treatment is ineffective. Emergency exploratory laparotomy might be an effective method for the patients with acute lower digestive tract hemorrhage associated with hemorrhagic shock.

To explore the influencing factors vaginal birth after cesarean.

39 cases who succeeded in vaginal birth after cesarean were included as cases group. Controls, matched 2:1 to cases, were selected from patients who did not complete vaginal delivery. 12 relevant influencing factors were compared between the two groups by univariate and Logistic regression analysis.

There were significant differences between the two groups in operation indication of previous cesarean section, antenatal body mass index (BMI), age, postoperative fever of previous cesarean section, newborn weight, complication of pregnancy and lower uterine segment thickness by univariate analysis (P＜0.05). The result of Logistic regression analysis showed that there were significant differences between the two groups in antenatal body mass index (BMI), age, newborn weight, complication of pregnancy and lower uterine segment thickness (P＜0.05).

Antenatal body mass index (BMI), age, newborn weight, complication of pregnancy and lower uterine segment thickness could be assessed to screen the appropriate maternal in clinic concerning safety of the maternal and infant.

To investigate the application value of a new pancreaticojejunostomy method to prevent the postoperative pancreatic fistula of pancreaticoduodenectomy ( PD).

from June of 2009 to August of 2013, 38 PD patients were treated with pancreaticojejunostomy(pj) which used jejunal mucosal stripping with double purse string suture ligation and anastomotic pancreatic upper and lower edge suture ligation. Retrospective analysis of feasibility, efficacy and complications of the anastomosis.

38 cases of patients with PD and PJ were all alive. 2 case of pancreatic leakage, which occurred on the seventh day and the tenth day postoperative, daily drainage fluid volume about 20-100 ml, recovered with the use of somatostatin drug after 3 weeks, including 1 cases of pancreas head carcinoma, 1 cases of cholangiocarcinoma. The other patients recovered well, no abdominal infection and bleeding, no biliary and intestinal fistula, no other serious complications. No death case.

the PJ of jejunum mucosal stripping double purse string suture ligation and anastomotic pancreatic upper and lower edge suture ligation, which pancreatic fistula rate is low, is feasibility, simple, effective and reliable, have significant clinical benefit and practical value.

To observe the effect of atorvastatin and rosuvastatin on change of LDL-C, CX3CR1 and Hcy in coronary heart disease (CHD) patients after 4 weeks.

LDL-C were measured by the colorimetric method, the expression of CX3CR1 on monocytes were measured by direct immunofluorescence techniques associated with flow cytometer(FCM) and Hcy were measured by enzymatic cycling methods in 80 patients with stable angina pectoris(SAP) confimed by coronary arteriography, 80 patients with unstable angina pectoris(UAP). They Were randomly divided into two groups（A group and B group）, A group took atorvastatin and B group took rosuvastatin.

Compared with the data before treatment, atorvastatin and rosuvastatin both decreased the level of LDL-C, CX3CR1 and Hcy at the 4th weak all of the CHD groups, and the differences were statistically significant (P＜0.05). And there was significant difference between the treatment groups (P＜ 0.05).

Atorvastatin and Rosuvastatin all can significantly decrease serum lipid level and have an anti-inflammatory effect.But rosuvastatin can higher strengthen the effects on decreasing lipids than atorvastatin.

To investigate the expression and clinical significances of survivin in benign and malignant lesions of breast.

The expression of survivin are observed using immunochemistry method in 23 cases of normal tissue beside malignant lesion, 28 cases of breast proliferative lesions (including 20 usual ductal hyperplasia, 8 atypical ductal hyperplasia), 8 cases of intraductal carcinoma, 98 cases of invasive ductal breast carcinoma.

The expression of survivin in atypical ductal hyperplasia and intraductal carcinoma was higher than that in normal tissue (P＜0.01); 49.0% of invasive ductal breast carcinoma expressed survivin, also higher than normal tissue (P＜0.05). There was no statistical significance of survivin expression between usual ductal hyperplasia and intraductal carcinoma. The expression of survivin in invasive ductal breast carcinoma was correlative to the grade, metastasis in lymph node and the TNM staging. It did not show any correlation between the age, size of tumor, expression of ER and PR.

The expression of survivin varies in different lesions, survivin may play a important role in tumorigendsis and progession of breast carcinoma and its overexpression indicates worse prognosis, which might be new application in the diagnosis, treatment and prognosis of breast cancer.

To discuss the clinical effect of ambroxol in premature children with respiratory distress syndrome (NRDS).

The clinical data of 62 premature children in our hospital during April 2012 and April 2013 were collected, and they were randomly divided into observation group (39 cases) and control group (23 cases). The control group were treated with conventional treatment，the observation group were treated with ambroxol on the basis of conventional treatment, Observed the oxygen partial pressure, incidence of RDS, mechanical ventilation rate, duration of mechanical ventilation, oxygen therapy time and length of stay of two groups.

In the observation group, neonatal respiratory distress syndrome was significantly lower than that in the control group (P＜0.05). The oxygen therapy time ,mechanical ventilation rate and length of stay of observation group were shorter than that of the control group, the difference were statistically significant (P＜0.05).

Ambroxol in premature children NRDS has a good effect, simple, convenient, and is worthy of clinical application.

To review the practice on scaling up antiretroviral therapy (ART) among people living with HIV/AIDS in Yunnan, China since 2011 and propose the relevant policy suggestions.

Through the Yunnan data analysis from National ART Baseline of the China Information System for Diseases Control and Prevention, official documents and the sitevisiting, to integrate these findings and trigger discussion on the sustainable ART expandation operation.

Since 2011 Yunnan explored to scale up ART program, taking the measures on decentralizing the ART to township health centers for more accessible locally, strengthening policy on more ART coverage, optimizing the ART regimens and encouraging the social organization involvement, the ART coverage on people with CD4 count below 350 had been rising, the people with CD4 above 350 had been accounting higher ratio in the increasing annual na?ve people with ART, and the primary analysis showed the good viral suppression and lower mortality among people with ART.

Though the initial practice has showed the effect, the stainable operation on the ART scale-up will resolve the critical problems.

To analyze the laparoscopic endometriosis fertility index in endometriosis (EMT) application results in infertility.

100 cases of uterine endometriosis infertility hospital merger in January 2011 admitted in June 2013, to give patients the laparoscopic surgery for patients with endometriosis fertility index (EFI) score, analyze postoperative pregnancy.

100 cases of patients after 3 years, 52 cases of successful pregnancies, the total pregnancy rate was 52% after the first year pregnancy rate was 31%, 16% the second year, third year is 5%. EFI score≤4 of 14 patients, three years, three cases of successful pregnancy, the pregnancy rate of 21.4%; 5-8 of 49 patients , three years, 21 cases of successful pregnancy, the pregnancy rate of 42.9%; 9-10 of 37 patients, 3 during the year 28 cases of successful pregnancy, the pregnancy rate 75.7%, EFI score 9-10 points pregnancy rate was significantly better than patients with other patients (P＜0.05).

Laparoscopic Endometriosis fertility index is an important indicator of the evaluation of endometriosis patients with infertility fertility, clinical patient specific treatment may be given in accordance with EFI score circumstances, improves patient pregnancy.

To explore the feasibility and effectiveness of follow up model of QQ group on patients with hyperuricemia.

120 patients with hyperuricemia found in health examination were divided randomly into intervention group and control group with each group of 60 cases. Both groups received same health education before follow up, afterwards, the control group received telephone follow up, while the intervention group received follow up of QQ group.

The behavior of following medical order of intervention group in diet, movement and controlling body weight were significantly better than the control(all P＜0.01) after intervention. After intervention of 3 and 6 months, the level of blood uric acid of two groups dropped obviously (all P＜0.01), but the intervention group was more significant than the control(both P＜ 0.01).

Follow up model of QQ group can obviously improve the behavior of following medical order, decrease the level of blood uric acid and the cost of follow up of patients with hyperuricemia, and it can be widely used in clinic.

Discuss laparoscopic uterine fibroids excluding secondary abdominal postoperative parasitic leiomyoma case. Method a retrospective study, summarize 1 case operation process.

The operations were successful, removed the mass pathology in leiomyoma.

Laparoscopic uterine flesh tumour removed is strong technical new treatments, laparoscopic uterine fibroids eliminate postoperative parasitic leiomyoma, is relatively rare. Therefore, to have a history of laparoscopy myoma eliminating should regularly review, and should pay attention to outside the uterus, abdominal cavity, if discover the abdominal cavity or solid neoplasm, should be alert to the possibility of secondary parasitic myoma.

To study the attitude to seeing doctors of elderly hospitalized patients and its influencing factors through cross- sectional study, in order to provide scientific and reliable basis for improving the health of elderly patients.

Select the elderly hospitalized patients from May 2012 to December 2012 in a general hospital as our research object, Investigate the social demographic characteristics, body health, nearly a year of health and the attitude of seeing doctors of the elderly hospitalized patients by the self-made questionnaire.

The survey shows that in the attitude to seeing doctors, having a positive attitude to seeing doctors accounted for 47.3% , having negative attitude to seeing doctors accounted for 52.7%. In active attitude reasons, there were five main reasons:“worrying about diseases becoming more and more serious”, “should see doctors as soon as possible when feeling no good”,“couldn’t endure the pain of diseases”,“seeing doctors are convenient”, "positive treatment can obtain the formal treatment". In negative attitude reasons, there were four main reasons: economic difficulties, thinking "seeing a doctor is very trouble, spending much time", "with no accompany" (28.4% ), and thinking "seeing a doctor is useless", think " it would also be good if not to see a doctor or take medicine". Residence, payment of medical expenses and physical examination situation were the influence factors of attitude to seeing doctors of elderly hospitalized patients.

The proportion of negative attitude elderly inpatients was higher in this hospital. We need to take further measures to improve their attitude with medical treatment.