Abstract: Objective To compare the clinical outcomes of embryos selected using time-lapse microscopy and traditional
morphological method in IVF/ICSI cycles and evaluate the clinical value of time-lapse microscopy in early embryo monitoring
and selection. Methods We retrospectively analyzed the clinical data of 139 IVF/ICSI cycles with embryo selection based on
time-lapse monitoring (TLM group, n=68) and traditional morphological method (control group, n=71). The βHCG-positive
rate, clinical pregnancy rate and embryo implantation rate were compared between the 2 groups. Subgroup analysis was
performed in view of female patients’age and the fertilization type. Results The βHCG-positive rate, clinical pregnancy rate
and implantation rate were 66.2%, 61.8% and 47.1% in TLM group, significantly higher than those in the control group (47.9%,
43.7% and 30.3%, respectively; P<0.05). Compared with patients below 30 years of age, patients aged between 31 and 35 years
benefited more from time-lapse monitoring with improved clinical outcomes. time-lapse monitoring significantly increased the
βHCG-positive rate, clinical pregnancy rate and implantation rate for patients undergoing IVF cycles, but not for those
undergoing ICSI or TESA cycles. Conclusion Compared with those selected using traditional morphological method, the
embryos selected with time-lapse microscopy have better clinical outcomes, especially in older patients (31-35 years of age)
and in IVF cycles.