Abstract: Objective To investigate the protective effects of Zhenwutang (ZWT) on cardiac function in mice with uremic
cardiomyopathy (UCM). Methods Thirty C57BL/6 mice were randomized into 3 equal groups, including a sham-operated
group, subtotal nephrectomy (UCM model) group and subtotal nephrectomy with ZWT treatment group. The mice in the
former two groups were treated with distilled water. The changes in cardiac functions, myocardial structure and renal function
of the mice were evaluated with echocardiography, HE staining and biochemical assay, respectively. Western blotting was used
to detect the expression level of adenosine monophosphate-activated protein kinase (AMPK) and mammalian target of
rapamycin (mTOR) in the heart tissues. Results Compared with the sham-operated group, the mice in the model group
showed significantly lowered body weight and increased heart weight, heart index, left ventricular posterior wall thickness in
diastole (LVPWd) and in systole (LVPWs), blood urea nitrogen (BUN) and serum creatinine (Scr) (P<0.05); Pathological
examination revealed myocardial hypertrophy in the model group with markedly decreased expression levels of p-AMPK and
significantly increased p-mTOR expression (P<0.05). ZWT treatment significantly decreased the heart weight, heart index,
LVPWd, and LVPWs and expression level of p-mTOR (P<0.05), increased the expression level of p-AMPK (P<0.05), and
obviously ameliorated histological injury of the heart in mice with UCM. Conclusion ZWT can protect the cardiac function in
mice with subtotal nephrectomy-induced UCM possibly via the AMPK-mTOR signal pathway.