Abstract: We proposed a new stitching method based on sift features to obtain an enlarged view of transmission electron
microscopic (TEM) images with a high resolution. The sift features were extracted from the images, which were then combined
with fitted polynomial correction field to correct the images, followed by image alignment based on the sift features. The
image seams at the junction were finally removed by Poisson image editing to achieve seamless stitching, which was validated
on 60 local glomerular TEM images with an image alignment error of 62.5 to 187.5 nm. Compared with 3 other stitching
methods, the proposed method could effectively reduce image deformation and avoid artifacts to facilitate renal biopsy
pathological diagnosis.