Abstract: Objective To investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and
osteopontin systhesis in vitro and explore the mechanisms. Methods HASMCs were treated with 10-100 nmol/L anti-miR-145,
and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes
in osteopontin synthesis after the treatment was quantified with Western blotting. Results Treatment with 10 and 50 nmol/L
anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L
anti-miR-145 obviously inhibited the cell apoptosis (P<0.01). Conclusion Anti-miR-145 promotes HASMC proliferation and
osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis
of airway remodeling.