Abstract: Objective To construct a RNA interference lentiviral vector for human glutathione peroxidase 2 (GPX2) gene and
observe the effect of GPX2 knockdown on cell apoptosis. Methods The sequence of the small interfering RNA (siRNA) for
GPX2 interference was inserted into the pSicoR vector. HepG2 cells were infected by the packaged si-GPX2 lentivirus and the
expression of GPX2 in the infected cells was detected by both RT-PCR and Western blotting. Changes of cell apoptosis
following the infection were analyzed by flow cytometry. Results The lentiviral particles pSicoR-GPX2 were successfully
packaged. The expression of GPX2 in the infected cells was obviously down-regulated at both RNA and protein levels. GPX2
knockdown caused increased apoptosis rate, increased Bax expression and lowered Bcl-2 expression in HepG2 cells.
Conclusion We have successfully constructed the lentiviral vector for RNA interference of human GPX2 gene.