Abstract: Objective To prepare and characterize a nano-scale fibrous hydrophilic poly-L-lactic acid/ Bioglass (PLLA/BG)
composite membrane and evaluate its biocompatibility as a composite membrane for guiding bone regeneration (GBR).
Methods PLLA/BG-guided bone regeneration membrane was treated by oxygen plasma to improved its hydrophilicity. The
growth of MG-63 osteoblasts on the membrane was observed using Hoechst fluorescence staining, and the biocompatibility of
the membrane was evaluated by calculating the cells adhesion rate and proliferation rate. Osteogenesis of MG-63 cells was
assessed by detecting alkaline phosphatase (ALP), and the formation of calcified nodules and cell morphology changes were
observed using scanning electron microscope (SEM). Results The cell adhesion rates of PLLA/BG-guided bone regeneration
membrane treated with oxygen plasma were (30.570±0.96)%, (47.27±0.78)%, and (66.78±0.69)% at 1, 3, and 6 h, respectively,
significantly higher than those on PLLA membrane and untreated PLLA/BG membrane (P<0.01). The cell proliferation rates on
the 3 membranes increased with time, but highest on oxygen plasma-treated PLLA/BG membrane (P<0.01). Hoechst
fluorescence staining revealed that oxygen plasma treatment of the PLLA/BG membrane promoted cell adhesion. The
membranes with Bioglass promoted the matrix secretion of the osteoblasts. Under SEM, the formation of calcified nodules and
spindle-shaped cell morphology were observed on oxygen plasma-treated PLLA/BG membrane. Conclusion Oxygen
plasma-treated PLLA/BG composite membrane has good biocompatibility and can promote adhesion, proliferation and
osteogenesis of the osteoblasts.