Abstract: Objective To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using
borate buffer. Methods BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of
uricase in the prepared BUCLPs were examined. Results The optimal temperature of BUCLP and URI was 40 ºC, their optimal
pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 μmol/L and 13.623 μmol/L, respectively.
Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase
binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm.
Conclusion Uricase activity is enhanced after loading in uricase and catalase liposomes.